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Product Info Summary
| SKU: | M06990 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NR2F6 Purified Monoclonal Antibody
SKU/Catalog Number
M06990
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NR2F6 Purified Monoclonal Antibody (Catalog# M06990). Tested in Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-NR2F6 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06990)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-51
Isotype
Mouse IgG2a kappa
Immunogen
Recombinant human NR2F6 protein expressed in E. coli. The mouse monoclonal antibody EM-51 recognizes NR2F6, a transcriptional repressor (intracellular antigen) expressed mainly in the heart, placenta, liver, skeletal muscle, kidney and pancreas, but also e.g. in T cell subpopulations.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M06990 is reactive to NR2F6 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
43.0 kDa
Background of NR2F6
NR2F6 (nuclear receptor subfamily 2 group F member 6), also known as EAR2 or ERBAL2, is a transcription factor involved in modulation of hormonal responses. NR2F6 represses e.g. transcription of the oxytocin-neurophysin gene, renin gene, lutropin-choriogonadotropic hormone receptor gene, and the thyroid hormone receptor gene. In the immune system, NR2F6 affects IL-17 expression in the Th-17 cells, thus also the balance between immunological tolerance and autoimmunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06990 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: Positive control: HEK293T/17/NR2F6/Ha-tag transfectants, negative control: HEK293T/17 cells.
Flow cytometry: 3-9 µg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Separation of human monocytes stained using anti-NR2F6 (EM-51) purified antibody (concentration in sample 3 µg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
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Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-NR2F6 (EM-51) purified antibody (concentration in sample 3 µg/ml, GAM APC).
Specific Publications For Anti-NR2F6 Purified Monoclonal Antibody (M06990)
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