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PCNA antibody (A00125) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 41 Publications

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a00125 pcna primary antibodies fcm testing 10

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Flow Cytometry analysis of 293T cells using anti-PCNA antibody (A00125).
Overlay histogram showing 293T cells stained with A00125 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCNA Antibody (A00125, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Anti-PCNA Antibody Picoband®

PCNA antibody

41 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

PCNA antibody was positively detected by WB in:

human Hela whole cell, human MCF-7 whole cell, human 293T whole cell, human HepG2 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

PCNA antibody was positively detected by IHC in:

human breast cancer tissue, human colorectal adenocarcinoma tissue, human tonsil tissue, human papillary thyroid carcinoma tissue, human glioblastoma tissue, human liver cancer tissue, rat colon tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

PCNA antibody was positively detected by ICC/IF in:

A431 cell

PCNA antibody was positively detected by FC in:

293T cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Bovine

Predicted

Zebrafish

Predicted

Product Information

Product Name

Anti-PCNA Antibody Picoband®
View all PCNA Antibodies

Form

Lyophilized

Uniprot

P12004

Gene ID

5111

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PCNA recombinant protein (Position: M1-S261).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

28.8 kDa

Background of PCNA

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Cite This Product

Anti-PCNA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00125)

Protocols

Western Blot protocol for PCNA antibody

Review Protocol

Immunohistochemistry (IHC) protocol for PCNA antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PCNA antibody

Review Protocol

Flow Cytometry (FC) protocol for PCNA antibody

Review Protocol

Publications

Inflachromene attenuates monocrotaline-induced pulmonary arterial hypertension by suppressing the HMGB1–TLR4/RAGE–NF-κB signaling pathway

Published: 2026-04-26

Journal: INTERNATIONAL IMMUNOPHARMACOLOGY

Application: WB

Impact Factor: 4.70

Species: Rat

PMID: 42044573

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Free Secondary Antibody

Anti-PCNA Antibody Picoband®

PCNA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Inflachromene attenuates monocrotaline-induced pulmonary arterial hypertension by suppressing the HMGB1–TLR4/RAGE–NF-κB signaling pathway

Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

GATA3-NLRP1 Axis Inhibits Hepatocellular Carcinoma Progression Through the Hedgehog Signaling

5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2

Tailoring Carrier-Free Nanoparticles based on Natural Small Molecule Assembly for Synergistic Anti-tumor Efficacy

CircLMBR1 inhibits phenotypic transformation of hypoxia-induced pulmonary artery smooth muscle via the splicing factor PUF60

Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis

Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes

HMGB1 May Modulates Proliferation and Migration of Endothelial Cells by YAP1 under Hypoxia

Forkhead Box P4 promotes the proliferation of cells in colorectal adenocarcinoma

Expression of eIF6 and its relationship with cell proliferation in colorectal adenocarcinoma

Hepatocyte-targeted delivery using oleanolic acid-loaded liposomes for enhanced hepatocellular carcinoma therapy

Grape Seed Proanthocyanidins (GSPs) Inhibit the Development of Cutaneous Squamous Cell Carcinoma by Regulating the hsa_circ_0070934/miR-136-5p/PRAF2 Axis

MicroRNA153 induces apoptosis by targeting NFATc3 to improve vascular remodeling in pulmonary hypertension

1H-NMR based metabolic study of MMTV-PyMT mice along with pathological progress to screen biomarkers for the early diagnosis of breast cancer

Bioactive constituents of Mosla chinensis-cv. Jiangxiangru ameliorate inflammation through MAPK signaling pathways and modify intestinal microbiota in DSS-induced colitis mice

Human Exosomes Accelerate Cutaneous Wound Healing by Promoting Collagen Synthesis in a Diabetic Mouse Model

LIN28A inhibits DUSP family phosphatases and activates MAPK signaling pathway to maintain pluripotency in porcine induced pluripotent stem cells

All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells

Development-promoting effect of chicken embryo membrane on chicken ovarian cortical pieces of different age

Puerarin prevents progression of experimental hypoxia-induced pulmonary hypertension via inhibition of autophagy

Anti-colorectal cancer biotargets and biological mechanisms of puerarin: Study of molecular networks

CircSETD3 (Hsa_circ_0000567) acts as a sponge for microRNA-421 inhibiting hepatocellular carcinoma growth

Synergistic inhibitory effects of capsaicin combined with cisplatin on human osteosarcoma in culture and in xenografts

Lipopolysaccharide-induced proliferation and glycolysis in airway smooth muscle cells via activation of Drp1

Calcimimetic R568 reduced the blood pressure and improved aortic remodeling in spontaneously hypertensive rats by inhibiting local renin‑angiotensin system activity

Enhanced drug delivery using sonoactivatable liposomes with membrane-embedded porphyrins

The enhanced antitumor-specific immune response with mannose- and CpG-ODN-coated liposomes delivering TRP2 peptide

Supramolecular cisplatin-vorinostat nanodrug for overcoming drug resistance in cancer synergistic therapy

Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

Novel perfusion-decellularized method to prepare decellularized ureters for ureteral tissue-engineered repair

Comparison of the synergistic effect of lipid nanobubbles and SonoVue microbubbles for high intensity focused ultrasound thermal ablation of tumors

Nanoparticle-enhanced synergistic HIFU ablation and transarterial chemoembolization for efficient cancer therapy

Pemetrexed Induces G1 Phase Arrest and Apoptosis through Inhibiting Akt Activation in Human Non Small Lung Cancer Cell Line A549

Icariin attenuates hypoxia-induced oxidative stress and apoptosis in osteoblasts and preserves their osteogenic differentiation potential in vitro

Comparison of posterior capsule opacification in rabbit eyes receiving different administrations of rapamycin

Study on mouse model of triple-negative breast cancer: association between higher parity and triple-negative breast cancer

Simvastatin Decreases Stent-Induced In-Stent Restenosis Rate Via Downregulating the Expression of PCNA and Upregulating that of p27kip1

Lateral hypothalamic area mediated the aggravated effect of microinjection of Baclofen into cerebellar fastigial nucleus on stress gastric mucosal damage in rats

Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma

Pancreas duodenal homeobox-1 expression and significance in pancreatic cancer

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