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Product Info Summary
| SKU: | M00474 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-OGT Rabbit Monoclonal Antibody
SKU/Catalog Number
M00474
BM5564 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-OGT Rabbit Monoclonal Antibody catalog # M00474. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OGT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00474)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEDG-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human OGT / O-Linked N-Acetylglucosamine Transferase Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Reactive Species
M00474 is reactive to OGT in Human, Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
116.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00474 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human Hela whole cell, human 293T whole cell, rat thymus tissue, rat spleen tissue, mouse thymus tissue, mouse spleen tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of OGT using anti-OGT antibody (M00474).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified monoclonal antibody (M00474) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OGT at approximately 110 kDa. The expected band size for OGT is at 117 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Specific Publications For Anti-OGT Rabbit Monoclonal Antibody (M00474)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-OGT Rabbit Monoclonal Antibody
Question
We have observed staining in rat erythroleukemia. Any tips? Is anti-OGT Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
From what I have seen in literature erythroleukemia does express OGT. From what I have seen in Uniprot.org, OGT is expressed in middle temporal gyrus, liver, endometrium, fetal brain spinal cord, colon pancreas, hepatoma, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have OGT expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Pancreas, Pubmed ID: 15489334
Endometrium, Fetal brain, and Spinal cord, Pubmed ID: 17974005
Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 12150998
Liver, Pubmed ID: 9083068
Boster Scientific Support
Answered: 2020-02-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain spinal cord using anti-OGT Monoclonal antibody M00474. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
Do you have a BSA free version of anti-OGT Monoclonal antibody M00474 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-OGT Monoclonal antibody M00474 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-OGT Monoclonal antibody M00474 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The anti-OGT Monoclonal antibody (M00474) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-26
Question
We want using your anti-OGT Monoclonal antibody for histone h4-k16 acetylation studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thanks for your inquiry. This M00474 anti-OGT Monoclonal antibody is validated on a549 cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-31
Question
Will M00474 anti-OGT Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Carter
Verified customer
Asked: 2019-09-23
Answer
It shows on the product datasheet, M00474 anti-OGT Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-23
Question
I see that the anti-OGT Monoclonal antibody M00474 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-17
Question
Does anti-OGT Monoclonal antibody M00474 work for WB with fetal brain spinal cord?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
According to the expression profile of fetal brain spinal cord, OGT is highly expressed in fetal brain spinal cord. So, it is likely that anti-OGT Monoclonal antibody M00474 will work for WB with fetal brain spinal cord.
Boster Scientific Support
Answered: 2019-08-21
Question
Is a blocking peptide available for product anti-OGT Monoclonal antibody (M00474)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
We do provide the blocking peptide for product anti-OGT Monoclonal antibody (M00474). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-05
Question
My colleagues were well pleased with the WB result of your anti-OGT Monoclonal antibody. However we have been able to see positive staining in endometrium isoform 4: cytoplasm. nucleus. using this antibody. Is that expected? Could you tell me where is OGT supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Based on literature, endometrium does express OGT. Generally OGT expresses in nucleus., isoform 2: mitochondrion, isoform 3: cytoplasm, isoform 4: cytoplasm. nucleus. Regarding which tissues have OGT expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Colon, and Pancreas, Pubmed ID: 15489334
Endometrium, Fetal brain, and Spinal cord, Pubmed ID: 17974005
Erythroleukemia, Pubmed ID: 23186163
Hepatoma, Pubmed ID: 12150998
Liver, Pubmed ID: 9083068
Boster Scientific Support
Answered: 2019-05-27
Question
My question regards to test anti-OGT Monoclonal antibody M00474 on rat fetal brain spinal cord for research purposes, then I may be interested in using anti-OGT Monoclonal antibody M00474 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The products we sell, including anti-OGT Monoclonal antibody M00474, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-17
Question
Please see the WB image, lot number and protocol we used for fetal brain spinal cord using anti-OGT Monoclonal antibody M00474. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-04-03
Question
My question regarding product M00474, anti-OGT Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00474 anti-OGT Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-03
Question
I was wanting to use your anti-OGT Monoclonal antibody for WB for rat fetal brain spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal brain spinal cord identification?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
You can see on the product datasheet, M00474 anti-OGT Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-30
Question
Is this M00474 anti-OGT Monoclonal antibody reactive to the isotypes of OGT?
N. Mitchell
Verified customer
Asked: 2017-12-15
Answer
The immunogen of M00474 anti-OGT Monoclonal antibody is A synthesized peptide derived from human OGT / O-Linked N-Acetylglucosamine Transferase Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-15
Question
We bought anti-OGT Monoclonal antibody for WB on liver a few months ago. I am using human, and We want to use the antibody for IF next. Our lab want to know about examining liver as well as endometrium in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Brown
Verified customer
Asked: 2014-01-15
Answer
I looked at the website and datasheets of our anti-OGT Monoclonal antibody and I see that M00474 has been validated on human in both WB and IF. Thus M00474 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-01-15
