Product Info Summary
SKU: | RP1080 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Osteopontin/SPP1 Antibody
View all Osteopontin/OPN Antibodies
SKU/Catalog Number
RP1080
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Osteopontin/SPP1 Antibody catalog # RP1080. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Osteopontin/SPP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1080)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1080 is reactive to SPP1 in Human, Mouse, Rat
Applications
RP1080 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
66 kDa, 25-55 kDa
Calculated molecular weight
35.423kDa
Background of Osteopontin/OPN
Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. And the encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. Also, this protein is a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, -
ELISA , 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: human SHG-44 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66,25-55KD. The expected band size for Osteopontin is at 35KD.
Protein Target Info & Infographic
Gene/Protein Information For SPP1 (Source: Uniprot.org, NCBI)
Gene Name
SPP1
Full Name
Osteopontin
Weight
35.423kDa
Superfamily
osteopontin family
Alternative Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin SPP1 BNSP, BSPI, ETA-1, OPN secreted phosphoprotein 1 osteopontin|SPP1/CALPHA1 fusion|early T-lymphocyte activation 1|nephropontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)|secreted phosphoprotein 1 variant 6|urinary stone protein|uropontin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPP1, check out the SPP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Osteopontin/SPP1 Antibody (RP1080)
Hello CJ!
RP1080 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li X,Hu B,Wang L,Xia Q,Ni X. P2X7 receptor-mediated phenotype switching of pulmonary artery smooth muscle cells in hypoxia. Mol Biol Rep.2021 Mar 1.doi:10.1007/s11033-021-06222-2. Epub ahead of print.PMID:33650080.
Species: Rat
RP1080 usage in article: APP:WB, SAMPLE:PASMCS, DILUTION:1:500
Caiyun Mu,Ye He,Yan Hu,Menghuan Li,Maowen Chen,Rong Wang,Yang Xiang, Zhong Luo,Kaiyong Cai,Construction of chemokine substance P-embedded biomimetic multilayer onto bioactive magnesium silicate-titanium implant for bone regeneration,Applied Materials Toda
Species: Mouse,Rat
RP1080 usage in article: APP:WB, SAMPLE:MSCS, DILUTION:NA
Chaowei Hu,Kun Zuo,Kuibao Li,Yuanfeng Gao,Mulei Chen,Roumu Hu,Ye Liu,Hongjie Chi,Hongjiang Wang,Yanwen Qin,Xiaoyan Liu,Jiuchang Zhong,Jun Cai,Xinchun Yang,Jing Li, "p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle
Species: Rat,Human
RP1080 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Icariin doped bioactive glasses seeded with rat adipose-derived stem cells to promote bone repair via enhanced osteogenic and angiogenic activities
Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in %u2026
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo
Shan A, Zhou C, He Y, Feng W, Chen G, Zhong G. Ren Fail. 2013;35(1):37-42. Doi: 10.3109/0886022X.2012.741645. Epub 2012 Nov 14. Expression Of Both Matrix Metalloproteinase-2 And Its Tissue Inhibitor-2 In Tunica Media Of Radial Artery In Uremic Pat...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Osteopontin/SPP1 Antibody
Question
We have observed staining in rat liver. Are there any suggestions? Is anti-Osteopontin/SPP1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
From what I have seen in literature liver does express SPP1. From what I have seen in Uniprot.org, SPP1 is expressed in amniotic fluid, liver, brain, kidney subthalamic nucleus, placenta, brain kidney, kidney, milk, pituitary, among other tissues. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2020-04-03
Question
We are currently using anti-Osteopontin/SPP1 antibody RP1080 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The anti-Osteopontin/SPP1 antibody (RP1080) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-27
Question
We have tried in the past anti-Osteopontin/SPP1 antibody for WB on milk in the past. I am using human, and We intend to use the antibody for ELISA next. I am interested in examining milk as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
I took a look at the website and datasheets of our anti-Osteopontin/SPP1 antibody and it seems that RP1080 has been tested on human in both WB and ELISA. Thus RP1080 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-06
Question
We are interested in using your anti-Osteopontin/SPP1 antibody for integrin cell surface interactions studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
C. Carter
Verified customer
Asked: 2014-02-13
Answer
I appreciate your inquiry. This RP1080 anti-Osteopontin/SPP1 antibody is validated on rat brain tissue, mouse brain. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-02-13
Question
My team were well pleased with the WB result of your anti-Osteopontin/SPP1 antibody. However we have been able to see positive staining in amniotic fluid secreted. using this antibody. Is that expected? Could you tell me where is SPP1 supposed to be expressed?
E. Banerjee
Verified customer
Asked: 2013-07-08
Answer
Based on literature, amniotic fluid does express SPP1. Generally SPP1 expresses in secreted. Regarding which tissues have SPP1 expression, here are a few articles citing expression in various tissues:
Brain, and Kidney, Pubmed ID: 15489334
Kidney, Pubmed ID: 1575754
Kidney, and Subthalamic nucleus, Pubmed ID: 14702039
Liver, Pubmed ID: 7945249, 24275569
Milk, Pubmed ID: 2736258, 15869464
Pituitary, Pubmed ID: 16807684
Placenta, Pubmed ID: 16303743
Boster Scientific Support
Answered: 2013-07-08