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ABCB4 antibody (PB9275) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HepG2 cells using anti-ABCB4 antibody (PB9275).
Overlay histogram showing HepG2 cells stained with PB9275 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody (PB9275, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with PB9275 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody (PB9275, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ABCB4 Antibody Picoband®
ABCB4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
ABCB4 antibody was positively detected by WB in:
human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCB4 antibody was positively detected by IHC in:
human liver cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
ABCB4 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-ABCB4 Antibody Picoband®
View all ABCB4 Antibodies
View all ABCB4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated Molecular Weight
141.5 kDa
Background of ABCB4
Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCB4 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. ABCB4 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.
Cite This Product
Anti-ABCB4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9275)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Mouse
80%
Moderate Homology
Rat
80%
Moderate Homology
Rabbit
78%
Moderate Homology
Dog
77%
Moderate Homology
Cow
66%
Low homology
Sheep
63%
Low homology
Zebrafish
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCB4 antibody
Immunohistochemistry (IHC) protocol for ABCB4 antibody
Flow Cytometry (FC) protocol for ABCB4 antibody
Review
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Submit A Review1 Review For Anti-ABCB4 Antibody Picoband®
Anti-ABCB4 Antibody (PB9275) clearly detected ABCB4 expression shifts in liver tissue from ANIT-induced cholestatic rats by WB, with only faint non-specific bands observed.
| SKU | PB9275 |
|---|---|
| Application | Western Blot |
| Sample | ANIT Model Rat Liver Tissue |
| Sample Processing Description | SD Rat Bile Stasis Liver Injury Induced by ANIT Gavage: Liver tissue was collected 48 h after induction, proteins were extracted using RIPA lysis buffer, quantified using a BCA Protein Assay Kit, and denatured by boiling after addition of Loading Buffer. |
| Other Reagents | Blocking Solution; RIPA Lysis Buffer |
| Primary Antibody | ABCB4 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody |
| Secondary Incubation | 1:50000 |
| Detection | ECL, Imaging system: Contact-based non-destructive imaging |
| Results Summary | The image shows the following lanes: Lane 1 represents the liver of a control group rat, displaying a clear and uniform ABCB4 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, showing a markedly increased ABCB4 band. This demonstrates that the antibody can effectively detect changes in ABCB4 expression. |
Yin Zhou, The Fifth Medical Center of PLA General Hospital
Verified customer
Submitted 2026-09-16
Product Promise
We promise Anti-ABCB4 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9275
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 22, 2026