Product Info Summary
SKU: | PA2090 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ABCB4 Antibody
View all MDR3/ABCB4 Antibodies
SKU/Catalog Number
PA2090
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ABCB4 Antibody catalog # PA2090. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2090)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ABCB4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2090 is reactive to ABCB4 in Human
Applications
PA2090 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
142 kDa
Calculated molecular weight
141.523kDa
Background of MDR3/ABCB4
Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. MDR1 and MDR3 constitute the human P-glycoprotein gene family. The MDR3 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. MDR3 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (PA2090).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PA2090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142 kDa. The expected band size for ABCB4 is at 142 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ABCB4 (Source: Uniprot.org, NCBI)
Gene Name
ABCB4
Full Name
Phosphatidylcholine translocator ABCB4
Weight
141.523kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC21; ABCB4; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; EC 3.6.3; EC 3.6.3.44; GBD1; MDR2; MDR3; MDR3MDR2/3; multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 3/multiple drug resistance 3; PFIC-3; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; PGY3; PGY3GBD1 ABCB4 ABC21, GBD1, ICP3, MDR2, MDR2/3, MDR3, PFIC-3, PGY3 ATP binding cassette subfamily B member 4 phosphatidylcholine translocator ABCB4|ATP-binding cassette sub-family B member 4|ATP-binding cassette, sub-family B (MDR/TAP), member 4|P-glycoprotein 3|P-glycoprotein-3/multiple drug resistance-3|multidrug resistance protein 3|multiple drug resistance 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCB4, check out the ABCB4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ABCB4 Antibody (PA2090)
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No publications found for PA2090
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