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Product Info Summary
| SKU: | A00960Y249-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-p130 Cas (phospho-Y249) BCAR1 Antibody
SKU/Catalog Number
A00960Y249-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p130 Cas (phospho-Y249) BCAR1 Antibody catalog # A00960Y249-1. Tested in WB,IHC,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p130 Cas (phospho-Y249) BCAR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00960Y249-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human p130 Cas around the phosphorylation site of Tyrosine 249.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00960Y249-1 is reactive to BCAR1 in Human, Mouse, Rat
Calculated molecular weight
93.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00960Y249-1 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of p-p130 Cas (Y249) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ML,30mins) Lane3:PC12 cell lysate treated with EGF(0.1ng/ML,30mins)
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Immunohistochemistry (IHC) analyzes of p-p130 Cas (Y249) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Specific Publications For Anti-p130 Cas (phospho-Y249) BCAR1 Antibody (A00960Y249-1)
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