Anti-p47 ING3 Antibody

Product Name

Anti-p47 ING3 Antibody

View all ING3 Antibodies

SKU/Catalog Number

A05458

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-p47 ING3 Antibody (Catalog # A05458). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-p47 ING3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05458)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 285-310 of Human ING3 protein (Inhibitor of growth family, member 3).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05458 is reactive to ING3 in Human

Applications

A05458 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

46.743kDa

Background of ING3

p47 ING3 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
WB: 1:200 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of ING3 expression in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. ING3 at 55KD was detected using goat anti-ING3 Antigen Affinity purified polyclonal antibody (Catalog # A05458) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1003).

Gene/Protein Information For ING3 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20089; ING2; inhibitor of growth family, member 3; inhibitor of growth protein 3; MEAF4; P47; p47ING3Eaf4 ING3 Eaf4, ING2, MEAF4, p47ING3 inhibitor of growth family member 3 inhibitor of growth protein 3

For more info on ING3, check out the ING3 Infographic

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In this infographic, you will see the following information for ING3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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