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Product Info Summary
| SKU: | A00001S15-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-p53 (phospho-S15) TP53 Antibody
SKU/Catalog Number
A00001S15-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-p53 (phospho-S15) TP53 Antibody catalog # A00001S15-1. Tested in WB,IHC,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p53 (phospho-S15) TP53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00001S15-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 15.
Reactive Species
A00001S15-1 is reactive to TP53 in Human, Mouse, Rat
Calculated molecular weight
43.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00001S15-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IP: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of p-p53 (S15) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine(0.2ng/ml,24h) Lane2:Raw264.7 cell lysate treated with colchicine(0.2ng/ml,24h) Lane3:H9C2 cell lysate treated with colchicine(0.2ng/ml,24h)
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Immunohistochemistry (IHC) analyzes of p-p53 (S15) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Western blot (WB) analysis of p-p53 (S15) pAb at 1:500 dilution Lane1:HEK293T treated with Etoposide(20ng/ml) for 2 hours whole cell lysate Lane2:HEK293T treated with Etoposide(20ng/ml) for 30 minutes whole cell lysate Lane3:HEK293T treated with Etoposide(20ng/ml) for 5 minutes whole cell lysate Lane4:HEK293T whole cell lysate(40ug)
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Immunoprecipitation of HepG2 cell lysates using p53 (phospho-S15) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using p53 (phospho-S15) pAb.
Specific Publications For Anti-p53 (phospho-S15) TP53 Antibody (A00001S15-1)
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