Anti-P Glycoprotein/ABCB1 Antibody

Product Name

Anti-P Glycoprotein/ABCB1 Antibody

View all TP53 Antibodies

SKU/Catalog Number

RP1034
PB0162 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

ABCB1 (P-glycoprotein; MDR1/PGY1) is an ATP-dependent ABC transporter and drug efflux pump with broad substrate specificity (xenobiotic transport), contributing to decreased intracellular drug accumulation in multidrug-resistant cells; page notes roles in resistance to anticancer drugs and as a transporter at the blood–brain barrier. Assay context: rabbit polyclonal IgG; IHC-validated; reactive species human/mouse/rat; immunogen is a synthetic peptide from the middle region of human P-glycoprotein; calculated MW 141.5 kDa; 100 µg/vial, lyophilized; reconstitution yields ~500 µg/mL; “no cross-reactivity with other proteins” stated. Often contextualized with immune/tumor microenvironment markers like CXCL10 (IP-10) and metabolic/immune modulators like CD73/NT5E (putative).

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P Glycoprotein/ABCB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1034)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1034 is reactive to TP53 in Human, Mouse, Rat

Calculated molecular weight

141.5 kDa

Background of TP53

P-GP, also called ABCB1 or PGY1, is a glycoprotein that in humans is encoded by the ABCB1 gene. It is mapped to 7q21.12. P-GP is a well-characterized ABC-transporter (which transports a wide variety of substrates across extra- and intracellular membranes) of the MDR/TAP subfamily. It is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent drug efflux pump with broad substrate specificity. P-GP is an ATP-dependent drug efflux pump forxenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood–brain barrier.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1034 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat

Positive Control

IHC: Human Lung Cancer Tissue, Mouse Kidney Tissue, Rat Kidney Tissue
IHC-F: Mouse Intestine Tissue, Rat Kidney Tissue

Validation Images & Assay Conditions

See full target information TP53

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