Product Info Summary
| SKU: | A01244 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-p57 CDKN1C Antibody
SKU/Catalog Number
A01244
Size
100μl
Form
Liquid
Description
Boster Bio Anti-p57 CDKN1C Antibody catalog # A01244. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p57 CDKN1C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01244)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p57Kip2 around the non-acetylation site of Lys278. AA range:241-290
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01244 is reactive to CDKN1C in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
32.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01244 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
Click image to see more details
Western Blot analysis of Jurkat cells using p57 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using p57Kip2 (Ab-278) Antibody.
Specific Publications For Anti-p57 CDKN1C Antibody (A01244)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-p57 CDKN1C Antibody
Question
Is there a BSA free version of anti-p57 antibody A01244 available?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-p57 antibody A01244 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-29
Question
Is this A01244 anti-p57 antibody reactive to the isotypes of CDKN1C?
G. Jha
Verified customer
Asked: 2019-11-04
Answer
The immunogen of A01244 anti-p57 antibody is Synthesized peptide derived from human p57 around the non-acetylation site of K278. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-04
Question
Will anti-p57 antibody A01244 work on pig WB with embryo?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
Our lab technicians have not tested anti-p57 antibody A01244 on pig. You can run a BLAST between pig and the immunogen sequence of anti-p57 antibody A01244 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig embryo in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-19
Question
I was wanting to use your anti-p57 antibody for WB for mouse metanephric glomerulus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse metanephric glomerulus identification?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
It shows on the product datasheet, A01244 anti-p57 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse metanephric glomerulus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-22
Question
Is a blocking peptide available for product anti-p57 antibody (A01244)?
W. Zhao
Verified customer
Asked: 2016-05-30
Answer
We do provide the blocking peptide for product anti-p57 antibody (A01244). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-05-30
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for metanephric glomerulus using anti-p57 antibody A01244. Let me know if you need anything else.
M. Walker
Verified customer
Asked: 2014-11-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-24

