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Product Info Summary
| SKU: | A02352 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Calcitonin/Calca Antibody
SKU/Catalog Number
A02352
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calcitonin/Calca Antibody catalog # A02352. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calcitonin/Calca Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02352)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Calcitonin, which shares 90.6% and 96.9% amino acid (aa) sequence identity with human and rat Calcitonin, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02352 is reactive to Calca in Human, Mouse, Rat
Calculated molecular weight
15.1 kDa
Background of Calca
Calcitonin, also known as CALCA, is a peptide hormone synthesized by the parafollicular cells of the thyroid. It is mapped to 11p15.2. Calcitonin belongs to the calcitonin-like protein family. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02352 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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IHC analysis of Calcitonin using anti-Calcitonin antibody (A02352).
Calcitonin was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Calcitonin Antibody (A02352) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of Calcitonin using anti-Calcitonin antibody (A02352).
Calcitonin was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Calcitonin Antibody (A02352) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Calcitonin/Calca Antibody (A02352)
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4 Customer Q&As for Anti-Calcitonin/Calca Antibody
Question
Is A02352 suitable for immunostaining of mouse long bone nerve fibers?
Verified customer
Asked: 2019-10-06
Answer
Yes, the Anti-Calcitonin/Calca Antibody Picoband™ (A02352) is suitable for the immunostaining of mouse long bone nerve fibers.
Boster Scientific Support
Answered: 2019-10-09
Question
Our team were well pleased with the WB result of your anti-Calcitonin/Calca antibody. However we have observed positive staining in thyroid carcinoma secreted. using this antibody. Is that expected? Could you tell me where is CALCA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
From literature, thyroid carcinoma does express CALCA. Generally CALCA expresses in secreted. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2019-09-25
Question
We have observed staining in mouse thyroid carcinoma. Are there any suggestions? Is anti-Calcitonin/Calca antibody supposed to stain thyroid carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
From literature thyroid carcinoma does express CALCA. From Uniprot.org, CALCA is expressed in dorsal root ganglion, thyroid carcinoma, pheochromocytoma, among other tissues. Regarding which tissues have CALCA expression, here are a few articles citing expression in various tissues:
Pheochromocytoma, Pubmed ID: 1318039
Thyroid carcinoma, Pubmed ID: 6610687
Boster Scientific Support
Answered: 2019-09-10
Question
We are currently using anti-Calcitonin/Calca antibody A02352 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
The anti-Calcitonin/Calca antibody (A02352) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-09
