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Product Info Summary
| SKU: | A07487 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PACRG Antibody
SKU/Catalog Number
A07487
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PACRG Antibody (Catalog # A07487). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-PACRG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07487)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human PACRG protein.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07487 is reactive to PACRG in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
33.3 kDa
Background of PACRG
PACRG (also known as Parkin coregulated gene protein and PARK2 coregulated) is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes. PACRG is expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow and is also expressed in heart, brain, skeletal muscle, kidney, lung and pancreas. PACRG is expressed in primary Schwann cells and very weakly by monocyte-derived macrophages, which are the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Splice variants have been described for this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07487 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:3,000
This affinity purified antibody has been tested for use in ELISA and western blot.
Validation Images & Assay Conditions
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Western blot using Boster's Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Specific Publications For Anti-PACRG Antibody (A07487)
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7 Customer Q&As for Anti-PACRG Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-PACRG antibody A07487. Let me know if you need anything else.
E. Zhao
Verified customer
Asked: 2020-02-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
See attached the WB image, lot number and protocol we used for heart left ventricle using anti-PACRG antibody A07487. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-PACRG antibody A07487 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-06
Question
I am looking for to test anti-PACRG antibody A07487 on human heart left ventricle for research purposes, then I may be interested in using anti-PACRG antibody A07487 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
The products we sell, including anti-PACRG antibody A07487, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-01
Question
Will anti-PACRG antibody A07487 work on canine WB with heart left ventricle?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
Our lab technicians have not validated anti-PACRG antibody A07487 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PACRG antibody A07487 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine heart left ventricle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-18
Question
Does anti-PACRG antibody A07487 work for WB with heart left ventricle?
N. Anderson
Verified customer
Asked: 2016-11-21
Answer
According to the expression profile of heart left ventricle, PACRG is highly expressed in heart left ventricle. So, it is likely that anti-PACRG antibody A07487 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2016-11-21
Question
We are currently using anti-PACRG antibody A07487 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
E. Wu
Verified customer
Asked: 2013-09-26
Answer
The anti-PACRG antibody (A07487) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-26
