Product Info Summary
SKU: | A07487 |
---|---|
Size: | 100ug |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-PACRG Antibody
SKU/Catalog Number
A07487
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-PACRG Antibody (Catalog # A07487). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-PACRG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07487)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human PACRG protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07487 is reactive to Pacrg in Mouse
Applications
A07487 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
27.726kDa
Background of Pacrg
PACRG (also known as Parkin coregulated gene protein and PARK2 coregulated) is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes. PACRG is expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow and is also expressed in heart, brain, skeletal muscle, kidney, lung and pancreas. PACRG is expressed in primary Schwann cells and very weakly by monocyte-derived macrophages, which are the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Splice variants have been described for this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PACRG expression in human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). PACRG at 40KD was detected using rabbit anti-PACRG Antigen Affinity purified polyclonal antibody (Catalog # A07487) at 1:1500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For Pacrg (Source: Uniprot.org, NCBI)
Gene Name
Pacrg
Full Name
Parkin coregulated gene protein homolog
Weight
27.726kDa
Alternative Names
FLJ32724; Glup; HAK005771; Molecular chaperone/chaperonin-binding protein; PARK2 coregulated gene protein; PARK2 co-regulated; PARK2CRG; parkin coregulated gene protein; parkin co-regulated gene protein; RP3-495O10.2 Pacrg|1700008H23Rik|PARK2 co-regulated|parkin coregulated gene protein homolog|PARK2 coregulated gene protein|hypertension-related protein 1-like protein|parkin co-regulated gene protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Pacrg, check out the Pacrg Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Pacrg: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PACRG Antibody (A07487)
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No publications found for A07487
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-PACRG Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart left ventricle using anti-PACRG antibody A07487. Let me know if you need anything else.
E. Zhao
Verified customer
Asked: 2020-02-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
See attached the WB image, lot number and protocol we used for heart left ventricle using anti-PACRG antibody A07487. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
I see that the anti-PACRG antibody A07487 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-06
Question
I am looking for to test anti-PACRG antibody A07487 on human heart left ventricle for research purposes, then I may be interested in using anti-PACRG antibody A07487 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-01
Answer
The products we sell, including anti-PACRG antibody A07487, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-01
Question
Will anti-PACRG antibody A07487 work on canine WB with heart left ventricle?
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
Our lab technicians have not validated anti-PACRG antibody A07487 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PACRG antibody A07487 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine heart left ventricle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-18
Question
Does anti-PACRG antibody A07487 work for WB with heart left ventricle?
N. Anderson
Verified customer
Asked: 2016-11-21
Answer
According to the expression profile of heart left ventricle, PACRG is highly expressed in heart left ventricle. So, it is likely that anti-PACRG antibody A07487 will work for WB with heart left ventricle.
Boster Scientific Support
Answered: 2016-11-21
Question
We are currently using anti-PACRG antibody A07487 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
E. Wu
Verified customer
Asked: 2013-09-26
Answer
The anti-PACRG antibody (A07487) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-26