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- Table of Contents
PTH1R antibody (A01588) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of HEL cells using anti-Parathyroid Hormone Receptor 1/PTH1R antibody (A01588).
Overlay histogram showing HEL cells stained with A01588 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Parathyroid Hormone Receptor 1/PTH1R Antibody (A01588, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEL cells stained with A01588 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Parathyroid Hormone Receptor 1/PTH1R Antibody (A01588, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
PTH1R antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
PTH1R antibody was positively detected by WB in:
human 293T whole cell, human HepG2 whole cell, human HUH-7 whole cell, monkey COS-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTH1R antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Tested
Recommend
Dog
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Rabbit
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Product Information
Product Name
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband®
View all PTH1R Antibodies
View all PTH1R Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Parathyroid Hormone Receptor 1/PTH1R recombinant protein (Position: R102-L446).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
66 kDa
Calculated Molecular Weight
66.4 kDa
Background of PTH1R
Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as PTH1R, PTHR, is a protein that in humans is encoded by the PTH1R gene. PTHR is a member of a family of G protein-coupled receptors. PTHR gene was mapped to the vicinity of the 3p21.3-p21.2 boundary by PCR analysis of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. PTH1R functions as a receptor for parathyroid hormone and for parathyroid hormone-related protein.
Cite This Product
Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01588)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
99%
High Homology
Monkey
99%
High Homology
Cow
98%
High Homology
Sheep
97%
High Homology
Rabbit
95%
High Homology
Mouse
93%
High Homology
Rat
93%
High Homology
Zebrafish
75%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PTH1R antibody
Flow Cytometry (FC) protocol for PTH1R antibody
Publications
Application:
Species:
TGF-β Induces Degradation of PTHrP Through Ubiquitin-Proteasome System in Hepatocellular Carcinoma
Published: 2015-04-05
Journal: Journal of Cancer
Application: IHC
Impact Factor: 3.27
Species: Human
PMID: 26000041
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Product Promise
We promise Anti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01588
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026