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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-NOX2/gp91phox/CYBB Antibody Picoband™
Boster Bio Anti-NOX2/gp91phox/CYBB Antibody Picoband™ catalog # A00328. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOX2/gp91phox/CYBB Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00328)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00328 is reactive to CYBB in Human, Mouse, Rat
A00328 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NOX2 using anti-NOX2 antibody (A00328).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG cell lysate,
Lane 2: human Hela cell lysate,
Lane 3: human HepG2 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified polyclonal antibody (Catalog # A00328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.
Gene/Protein Information For CYBB (Source: Uniprot.Org, NCBI)
Cytochrome b-245 heavy chain
CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CYBB, check out the CYBB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CYBB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00328 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
A00328 usage in article: APP:WB, SAMPLE:KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA
Obligatory role for GPER in cardiovascular aging and disease^
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity
Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?
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