Anti-NOX2/gp91phox/CYBB Antibody

SKU PA1667
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC-P, WB

Overview

Product Name Anti-NOX2/gp91phox/CYBB Antibody
SKU/Catalog Number PA1667
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain(CYBB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-NOX2/gp91phox/CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1667)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Anti-NOX2/gp91phox antibody, PA1667, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: SMMC Cell Lysate

Anti-NOX2/gp91phox antibody, PA1667, IHC(P)
IHC(P): Human Intestinal Cancer Tissue

Figure 3. Western blot analysis of NOX2 using anti- NOX2 antibody (PA1667).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: rat brain tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- NOX2 antigen affinity purified polyclonal antibody (Catalog # PA1667) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04839
Gene Name CYBB
Protein Name Cytochrome b-245 heavy chain
Tissue Specificity Detected in neutrophils (at protein level). .
Alternative Names Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b(558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB;NOX2;
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Molecular Weight 65336 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling).

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Polyclonal antibody for NOX2/CYBB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NOX2/CYBB information: Molecular Weight: 65336 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein; Tissue Specificity: Detected in neutrophils (at protein level).
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In stock
Order Product
PA1667
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Obligatory role for GPER in cardiovascular aging and disease^
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity
Du Z, Yang Q, Liu L, Li S, Zhao J, Hu J, Liu C, Qian D, Gao C. Neuroscience. 2015 Feb 12;286:281-92. Doi: 10.1016/J.Neuroscience.2014.11.061. Epub 2014 Dec 8. Nadph Oxidase 2-Dependent Oxidative Stress, Mitochondrial Damage And Apoptosis In The Ve...
Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?
Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...
Liao Y, Liu P, Guo F, Zhang Zy, Zhang Z. Plos One. 2013 Jul 24;8(7):E68963. Doi: 10.1371/Journal.Pone.0068963. Print 2013. Oxidative Burst Of Circulating Neutrophils Following Traumatic Brain Injury In Human.

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to gp91 phox antibody, gp91phox antibody, gp91-phox antibody, nox2 antibody
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