|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-NOX2/gp91phox Antibody|
|Description||Rabbit IgG polyclonal antibody for Cytochrome b-245 heavy chain(CYBB) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-NOX2/gp91phox Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1667)|
|Replacement Item||This antibody may replace the following items: sc-130543|sc-130548|sc-130549|sc-20782|sc-27635|sc-27636|sc-27637|sc-5826|sc-5827|sc-74514 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human NOX2(442-459aa YWLCRDTHAFEWFADLLQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cytochrome b-245 heavy chain|
|Molecular Weight||65336 MW|
|Protein Function||Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.|
|Tissue Specificity||Detected in neutrophils (at protein level). .|
|Sequence Similarities||Contains 1 FAD-binding FR-type domain.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Alternative Names||Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b(558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB;NOX2;|
|Research Areas|||immunology|adaptive immunity|t cells|cytotoxic cells| cancer|cancer metabolism|cellular metabolic process| metabolism|types of disease||
Background for Cytochrome b-245 heavy chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NOX2/gp91phox Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NOX2/gp91phox Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: SMMC Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
"This NOX2 antibody is highly specific. Highly recommended!"
--RAHUL KUMAR, PHARMACEUTICAL SCIENCES, UNIVERSITY OF COLORADO DENVER, POST DOC
|Sample||22RV1 cytosolic and membrane fractions|
"We used this antibody to study the inhibitory effect of GPE on hypoxia induced translocation of gp91phox subunit from cytosol to membrane in our recently published paper (Scientific Reports 6, Article number: 23135 (2016); doi:10.1038/srep23135). This antibody is very much specific in our in vitro system."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,