Anti-PARC CUL9 Antibody

Product Name

Anti-PARC CUL9 Antibody

View all CUL9 Antibodies

SKU/Catalog Number

A08481-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PARC CUL9 Antibody (Catalog # A08481-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

PARC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PARC CUL9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08481-1)

Host

Rabbit

Contents

PARC Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PARC antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PARC. The immunogen is located within the last 50 amino acids of PARC.

Reactive Species

A08481-1 is reactive to CUL9 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

281.2 kDa

Background of CUL9

The continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08481-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PARC antibody can be used for the detection of PARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CUL9

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