Anti-Hu CD53 Purified Monoclonal Antibody

Product Name

Anti-Hu CD53 Purified Monoclonal Antibody

View all CD53 Antibodies

SKU/Catalog Number

M06249-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD53 Purified Monoclonal Antibody (Catalog# M06249-1). Tested in Flow Cytometry, IP, WB, IHC-F application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD53 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06249-1)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-53

Isotype

Mouse IgG1

Immunogen

Leukocytes of pacient suffering from a LGL-type leukemia. The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

Cross-reactivity

Cross-reactivity with CLIC5b was not determined.

Reactive Species

M06249-1 is reactive to CD53 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

24.3 kDa

Background of CD53

CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M06249-1 is guaranteed for Flow Cytometry, IP, IHC-F, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors.
Flow cytometry: 1-5 μg/ml.

Validation Images & Assay Conditions

See full target information CD53

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