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Product Info Summary
| SKU: | PB9308 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PARK7/DJ1 Antibody Picoband®
SKU/Catalog Number
PB9308
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PARK7/DJ1 Antibody Picoband® catalog # PB9308. Tested in IP, IHC, ICC/IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PARK7/DJ1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9308)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9308 is reactive to PARK7 in Human, Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
19.9 kDa
Background of PARK7
Parkinson disease (autosomal recessive, early onset) 7, also known as DJ1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. PARK7 is mapped to chromosome 1p36. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. This gene may also function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that PARK7 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9308 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Positive Control
WB: human 293T whole cell, human PANC-1 whole cell, human Hela whole cell, human U251 whole cell, rat kidney tissue, mouse kidney tissue
ICC/IF: SK-OV-3 cell
IP: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of PARK7 using anti-PARK7 antibody (PB9308).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARK7 antigen affinity purified polyclonal antibody (Catalog # PB9308) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARK7 at approximately 20 kDa. The expected band size for PARK7 is at 20 kDa.
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Anti-PARK7 Picoband antibody, PB9308, IHC(P)
IHC(P): Mouse Pancreas Tissue
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Anti-PARK7 Picoband antibody, PB9308, IHC(P)
IHC(P): Rat Pancreas Tissue
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Anti-PARK7 Picoband antibody, PB9308, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue
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IF analysis of PARK7 using anti-PARK7 antibody (PB9308).
PARK7 was detected in an immunocytochemical section of SK-OV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PARK7 Antibody (PB9308) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunoprecipitating (IP) PARK7 in Hela whole cell lysate.
Western blot analysis of PARK7 using anti-PARK7 antibody (PB9308);
Lane 1: Hela whole cell lysates (30ug);
Lane 2: Rabbit control IgG instead of anti-PARK7 antibody in Hela whole cell lysate;
Lane 3: anti-PARK7 antibody (2μg) + Hela whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PARK7 antigen affinity purified polyclonal antibody (PB9308) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PARK7 at approximately 20 kDa. The expected band size for PARK7 is at 20 kDa.
Specific Publications For Anti-PARK7/DJ1 Antibody Picoband® (PB9308)
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1 Customer Q&As for Anti-PARK7/DJ1 Antibody Picoband®
Question
We are currently using anti-PARK7/DJ1 antibody PB9308 for rat tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
D. Miller
Verified customer
Asked: 2018-02-09
Answer
The anti-PARK7/DJ1 antibody (PB9308) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-09
