Anti-LRFN1 Antibody

Product Name

Anti-LRFN1 Antibody

View all LRFN1 Antibodies

SKU/Catalog Number

A13222

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-LRFN1 Antibody (Catalog # A13222). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

LRFN1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-LRFN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13222)

Host

Rabbit

Contents

LRFN1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

LRFN1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LRFN1. The immunogen is located within amino acids 700 - 750 of LRFN1.

Cross-reactivity

This antibody is predicted to not cross-react with other members of the LRFN family.

Reactive Species

A13222 is reactive to LRFN1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

82.3 kDa

Background of LRFN1

LRFN1 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while Lrfn2 and Lrfn5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system. At least two isoforms of LRFN1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A13222 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

LRFN1 antibody can be used for detection of LRFN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LRFN1

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