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- Table of Contents
GDNF antibody (PA1465) performs well in IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for GDNF? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GDNF Western Blot GuideBundle Save
Anti-GDNF Antibody Picoband®
GDNF antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | IHC, WB |
Tested Applications
GDNF antibody was positively detected by WB in:
recombinant human GDNF protein
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GDNF antibody was positively detected by IHC in:
human stomach cancer tissue, human lung cancer tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Human
Tested
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-GDNF Antibody Picoband®
View all GDNF Antibodies
View all GDNF Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GDNF, different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
23.7 kDa
Background of GDNF
GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.
Cite This Product
Anti-GDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1465)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Rat
88%
Moderate Homology
Mouse
82%
Moderate Homology
Zebrafish
76%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GDNF antibody
Immunohistochemistry (IHC) protocol for GDNF antibody
Publications
Application:
Species:
Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota
Published: 2022-11-26
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.03
Species: Mouse
PMID: 36511265
SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells
Published: 2022-03-27
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: IHC
Impact Factor: 9.43
Species: Rat
PMID: 35351151
Culture bovine prospermatogonia with 2i medium
Published: 2021-03-24
Journal: ANDROLOGIA
Application: IHC
Impact Factor: 2.78
Species: Bovine
PMID: 33763906
The interplay of BDNF-TrkB with NMDA receptor in propofol-induced cognition dysfunction
Published: 2018-04-05
Journal: BMC Anesthesiology
Application: IHC
Impact Factor: 1.79
Species: Rat
PMID: 29621970
The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro
Published: 2017-06-07
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.74
Species: Rat
PMID: 28680451
Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction
Published: 2013-08-08
Journal: Neural Regeneration Research
Application: IHC
Impact Factor: 0.14
Species: Rat
PMID: 25206528
Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey
Published: 2008-07-19
Journal: Neuroscience Bulletin
Application: IHC
Impact Factor: 0.00
Species: Monkey
PMID: 18500387
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Product Promise
We promise Anti-GDNF Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PA1465
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 20, 2026