Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 4 publication(s).
Anti-GDNF Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-GDNF Antibody
See all GDNF primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for GDNF detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GDNF information: Molecular Weight: 23720 MW; Subcellular Localization: Secreted ; Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.|
|Cite This Product||Anti-GDNF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1465)|
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|Anti-GDNF Antibody may replace the following items: sc 32551|sc 328|sc 13147|sc 9010|sc 398555|sc 328 G.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GDNF (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2668|
|Species Of This Entry||Human|
|Protein Name||Glial cell line-derived neurotrophic factor|
|Alternative Names||GDNF|Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3|
|Post Tranlational Modifications||Phosphorylation; Reduction; Cleavage; Glycosylation; Methylation; Acetylation; Ubiquitination; Iodination; Biotinylation; Oxidation; Myristoylation; Phosphorothioation; Dephosphorylation; Sulphation; Deamination; Gpi Anchoring; Ribosylation; Deglycosylation|
|Gene Location||5p13.2, on Chromasome 5, gene sequence: NC_000005.10 (37812677..37840044, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GDNF (Source: Uniprot.org, NCBI)
|Protein Function||Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.|
|Tissue Specificity||In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.|
|Related Diseases||Parkinson Disease; Nervousness; Neurodegenerative Disorders; Nerve Degeneration; Secondary Parkinson Disease; Neoplasms; Hirschsprung Disease; Ischemia; Pain; Spinal Cord Injuries; Inflammation; Nerve Damage; Sclerosis; Amyotrophic Lateral Sclerosis; Malignant Neoplasms; Brain Injuries; Primary Lateral Sclerosis; Crest Syndrome; Neuroblastoma; Tissue Adhesions|
|Related Pathways||Regeneration; Cell Death; Neuroprotection; Innervation; Secretion; Pathogenesis; Cell Proliferation; Kidney Development; Transport; Spermatogenesis; Localization; Cell Adhesion; Neurogenesis; Cell Migration; Aging; System Development; Programmed Cell Death; Reverse Transcription; Cell Differentiation; Dopamine Uptake|
|Background||GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.|
|Scientific References||PMID: 8493557 by Lin L.-F.H., et al. GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.|
PMID: 7867768 by Schaar D.G., et al. Multiple astrocyte transcripts encode nigral trophic factors in rat and human.
Our Boster Quality Guarantee for Anti-GDNF Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Recombinant Human GDNF Protein 10ng
Lane 2: Recombinant Human GDNF Protein 5ng
Lane 3: Recombinant Human GDNF Protein 2.5ng
Lane 4: Recombinant Human GDNF Protein 1.25ng
IHC(P): Rat Brain Tissue
IHC(P): Human Lung Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 4
|Zhang X, Shi K, Li Y, Zhang H, Hao J. Reprod Toxicol. 2014 Jun;45:87-93. Doi: 10.1016/J.Reprotox.2014.01.009. Epub 2014 Feb 4. Lipopolysaccharide Inhibits The Self-Renewal Of Spermatogonial Stem Cells In Vitro Via Downregulation Of Gdnf Expression...||PubMed ID 24503145|
|Li R, Xia W, Zhang Z, Wu K. Plos One. 2011;6(6):E21663. Doi: 10.1371/Journal.Pone.0021663. Epub 2011 Jun 27. S100B Protein, Brain-Derived Neurotrophic Factor, And Glial Cell Line-Derived Neurotrophic Factor In Human Milk.||PubMed ID 21738758|
|Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction||PubMed ID 25206528|
|Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells||PubMed ID 29925387|
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Note: sample size takes longer to process, average 5-7 days.
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