Anti-GDNF Antibody Picoband®

SPION-mediated magnetic actuation upregulates the expression of neurotrophic factors associated with repair phenotypes in Schwann cells. The protein expression of repair phenotype-related neurotrophic factors BDNF, GDNF, Olig1 and VEGF in different experimental groups was detected by immunohistochemical staining at 3 (a) , 7 (d) , 14 (g) and 21 days (j) after crush injury, and the protein expression levels were quantitatively analyzed ( b , e , h , k ). c , f , i , l The protein expression levels of such neurotrophic factors at the above time points were detected by ELISA, and the results were consistent with the immunohistochemical analysis. Each experiment was carried out in triplicate. The values are represented as the mean ± SD. Scale bar = 50 µm in panels a , d , g , j . * P < 0.05, ** P < 0.01
Index in PubMed under a CC BY license. PMID: 35351151

Western blot analysis of GDNF using anti-GDNF antibody (PA1465).
Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human GDNF protein 10 ng,
Lane 2: recombinant human GDNF protein 5 ng,
Lane 3: recombinant human GDNF protein 2.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody (Catalog # PB9064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDNF at approximately 17 kDa.

IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of GDNF using anti-GDNF antibody (PA1465).
GDNF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.