SKU PA1465
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC-P, WB

Overview

Product Name Anti-GDNF Antibody
SKU/Catalog Number PA1465
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-GDNF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1465)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human GDNF(142-158aa FRYCSGSCDAAETTYDK), different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

Anti-GDNF antibody, PA1465, Western blotting
Lane 1: Recombinant Human GDNF Protein 10ng
Lane 2: Recombinant Human GDNF Protein 5ng
Lane 3: Recombinant Human GDNF Protein 2.5ng
Lane 4: Recombinant Human GDNF Protein 1.25ng

Anti-GDNF antibody, PA1465, IHC(P)
IHC(P): Rat Brain Tissue

Anti-GDNF antibody, PA1465, IHC(P)
IHC(P): Human Lung Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P39905
Gene Name GDNF
Protein Name Glial cell line-derived neurotrophic factor
Tissue Specificity In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .
Alternative Names Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF;
Subcellular Localization Secreted .
Molecular Weight 23720 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands(GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization(FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET(rearranged during transfection) protooncogene.

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Polyclonal antibody for GDNF detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. GDNF information: Molecular Weight: 23720 MW; Subcellular Localization: Secreted ; Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.
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In stock
Order Product
PA1465
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

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Publications

Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells
Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction
Li R, Xia W, Zhang Z, Wu K. Plos One. 2011;6(6):E21663. Doi: 10.1371/Journal.Pone.0021663. Epub 2011 Jun 27. S100B Protein, Brain-Derived Neurotrophic Factor, And Glial Cell Line-Derived Neurotrophic Factor In Human Milk.
Zhang X, Shi K, Li Y, Zhang H, Hao J. Reprod Toxicol. 2014 Jun;45:87-93. Doi: 10.1016/J.Reprotox.2014.01.009. Epub 2014 Feb 4. Lipopolysaccharide Inhibits The Self-Renewal Of Spermatogonial Stem Cells In Vitro Via Downregulation Of Gdnf Expression...

Customer Q&As

  • Q: Is PA1465 able to detect preGDNF and mature GDNF?
    A: PA1465 is only able to detect mature GDNF
  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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