|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-PBP Antibody|
|Description||Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse;Rat.|
|Cite This Product||Anti-PBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1713)|
|Replacement Item||This antibody may replace the following items: sc-101504|sc-135778|sc-135779|sc-28837|sc-32623|sc-365973|sc-376925|sc-5422|sc-5423|sc-5424|sc-5426 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Phosphatidylethanolamine-binding protein 1(PEBP-1)|
|Molecular Weight||20830 MW|
|Protein Function||Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity). .|
|Tissue Specificity||HCNP is expressed in brain. Increased expression in aged senescence-accelerated mice.|
|Alternative Names||Phosphatidylethanolamine-binding protein 1;PEBP-1;HCNPpp;Hippocampal cholinergic neurostimulating peptide;HCNP;Pebp1;Pbp, Pebp;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases| cell biology|cell division|spindle| neuroscience|neurotransmitter|acetylcholine|neuropeptides|more neuropeptides| epigenetics and nuclear signaling| cancer|oncoproteins/suppressors|tumor suppressors||
Background for Phosphatidylethanolamine-binding protein 1(PEBP-1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-PBP Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-PBP Antibody Images
Click the images to enlarge.
All lanes: Anti PBP (PA1713) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,