Anti-CD146/MCAM Antibody

Boster Bio Anti-CD146/MCAM Antibody catalog # RP1050. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1050
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-CD146/MCAM Antibody

See all MCAM/CD146 products

SKU/Catalog Number

RP1050

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD146/MCAM Antibody catalog # RP1050. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD146/MCAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1050)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1050 is reactive to MCAM in Human

Applications

RP1050 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For MCAM (Source: Uniprot.Org, NCBI)

Uniprot ID

P43121

Gene ID

4162

Gene Name

MCAM

Full Name

Cell surface glycoprotein MUC18

Weight

71.607kDa

Alternative Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated antigen|cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated antigen A32|melanoma-associated antigen MUC18

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MCAM, check out the MCAM Infographic

MCAM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MCAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1050

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16 Customer Q&As for Anti-CD146/MCAM Antibody

Question

We have seen staining in human trachea. Are there any suggestions? Is anti-CD146/MCAM antibody supposed to stain trachea positively?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

From literature trachea does express MCAM. From Uniprot.org, MCAM is expressed in popliteal artery, melanoma, trachea, aortic endothelium, pns, cervix carcinoma, liver, among other tissues. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-02

Question

Is there a BSA free version of anti-CD146/MCAM antibody RP1050 available?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD146/MCAM antibody RP1050 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-23

Question

Would anti-CD146/MCAM antibody RP1050 work for WB with popliteal artery?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

According to the expression profile of popliteal artery, MCAM is highly expressed in popliteal artery. So, it is likely that anti-CD146/MCAM antibody RP1050 will work for WB with popliteal artery.

Boster Scientific Support

Answered: 2019-12-23

Question

I was wanting to use your anti-CD146/MCAM antibody for WB for human popliteal artery on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human popliteal artery identification?

Verified Customer

Verified customer

Asked: 2019-10-22

Answer

You can see on the product datasheet, RP1050 anti-CD146/MCAM antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human popliteal artery in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-22

Question

I see that the anti-CD146/MCAM antibody RP1050 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for popliteal artery using anti-CD146/MCAM antibody RP1050. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-22

Question

Will RP1050 anti-CD146/MCAM antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

You can see on the product datasheet, RP1050 anti-CD146/MCAM antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-04

Question

I have a question about product RP1050, anti-CD146/MCAM antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1050 anti-CD146/MCAM antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-25

Question

Is a blocking peptide available for product anti-CD146/MCAM antibody (RP1050)?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We do provide the blocking peptide for product anti-CD146/MCAM antibody (RP1050). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-23

Question

Is this RP1050 anti-CD146/MCAM antibody reactive to the isotypes of MCAM?

Verified Customer

Verified customer

Asked: 2019-02-07

Answer

The immunogen of RP1050 anti-CD146/MCAM antibody is E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-07

Question

I am interested in using your anti-CD146/MCAM antibody for cell adhesion studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-08-02

Answer

Thank you for your inquiry. This RP1050 anti-CD146/MCAM antibody is tested on a375 whole cell lysate, hela whole cell lysate, hepg2 whole cell lysate, lung cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-08-02

Question

I would like to test anti-CD146/MCAM antibody RP1050 on human popliteal artery for research purposes, then I may be interested in using anti-CD146/MCAM antibody RP1050 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Yang

Verified customer

Asked: 2018-01-24

Answer

The products we sell, including anti-CD146/MCAM antibody RP1050, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-24

Question

We are currently using anti-CD146/MCAM antibody RP1050 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?

S. Miller

Verified customer

Asked: 2017-10-25

Answer

The anti-CD146/MCAM antibody (RP1050) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-25

Question

We were satisfied with the WB result of your anti-CD146/MCAM antibody. However we have been able to see positive staining in popliteal artery membrane using this antibody. Is that expected? Could you tell me where is MCAM supposed to be expressed?

R. Taylor

Verified customer

Asked: 2016-09-09

Answer

Based on literature, popliteal artery does express MCAM. Generally MCAM expresses in membrane. Regarding which tissues have MCAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Liver, Pubmed ID: 19159218
Melanoma, Pubmed ID: 2602381, 8378324
PNS, Pubmed ID: 15489334
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-09-09

Question

Here is the WB image, lot number and protocol we used for popliteal artery using anti-CD146/MCAM antibody RP1050. Please let me know if you require anything else.

O. Roberts

Verified customer

Asked: 2015-04-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-04-02

Question

We purchased anti-CD146/MCAM antibody for IHC on popliteal artery last year. I am using human, and We intend to use the antibody for WB next. We want examining popliteal artery as well as aortic endothelium in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

V. Li

Verified customer

Asked: 2013-02-19

Answer

I have checked the website and datasheets of our anti-CD146/MCAM antibody and it seems that RP1050 has been tested on human in both IHC and WB. Thus RP1050 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-02-19

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RP1050
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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