Product Info Summary
SKU: | A02022-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-PCK1 Antibody
SKU/Catalog Number
A02022-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PCK1 Antibody catalog # A02022-2. Tested in ICC,IHC,WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02022-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ARTS-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02022-2 is reactive to PCK1 in Human
Applications
A02022-2 is guaranteed for IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69.195kDa
Background of PCK1
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
Hattori A., J. Biochem. 130:235-241(2001).
Nakajima D., DNA Res. 9:99-106(2002).
Clark H.F., Genome Res. 13:2265-2270(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ICC: 1:50-1:200
IHC: 1:50-1:200
WB: 1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of PCK1 using Anti-PCK1 Antibody (A02022-2).
ICC staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 2. Immunocytochemistry staining of PCK1 using Anti-PCK1 Antibody (A02022-2).
ICC staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For PCK1 (Source: Uniprot.org, NCBI)
Gene Name
PCK1
Full Name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Weight
69.195kDa
Superfamily
phosphoenolpyruvate carboxykinase [GTP] family
Alternative Names
EC 4.1.1.32; MGC22652; PCK1; PEPCK1; PEPCKC; PEPCK-C; PEPCK-CPEP carboxykinase; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic; Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase PCK1 PCKDC, PEPCK-C, PEPCK1, PEPCKC phosphoenolpyruvate carboxykinase 1 phosphoenolpyruvate carboxykinase, cytosolic [GTP]|PEP carboxykinase|phosphoenolpyruvate carboxykinase 1 (soluble)|phosphoenolpyruvate carboxykinase, cytosolic|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase|serine-protein kinase PCK1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PCK1, check out the PCK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCK1 Antibody (A02022-2)
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No publications found for A02022-2
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