Skip to the beginning of the images gallery

PCNA antibody (M00125-3) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 51 Publications

m00125 3 pcna primary antibodies fcm testing 10

Flow Cytometry analysis of JK cells using anti-PCNA antibody (M00125-3).
Overlay histogram showing JK cells stained with M00125-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCNA Antibody (M00125-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Bundle Save

Bundle & Save Program

Save up to 50% on Picoband® antibodies + earn 10% back on eligible partner reagents.

View Details

BUY MORE, SAVE MORE

This antibody qualifies!
2 = 20% off · 3 = 30% off · 4 = 40% off · 5+ = 50% off

View Details

Free Secondary Antibody

How to redeem? While adding to cart, choose a size with a FREE Secondary Antibody!

View Details

Anti-PCNA Antibody Picoband® (monoclonal, 2G2)

PCNA antibody

51 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Mouse	Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

PCNA antibody was positively detected by WB in:

human HEK293 whole cell, human Jurkat whole cell, human Colo320 whole cell, human K562 whole cell, rat spleen tissue, rat PC-12 whole cell, mouse spleen tissue, mouse RAW2647 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

PCNA antibody was positively detected by IHC in:

human clymphadenoma tissue, human colonic adenocarcinoma tissue, human gastric cancer tissue, human liver cancer tissue, human renal clear cell carcinoma tissue, mouse colon tissue, rat colon tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

PCNA antibody was positively detected by ICC/IF in:

HEP3B cell

PCNA antibody was positively detected by FC in:

JK cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Monkey

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Bovine

Predicted

Zebrafish

Predicted

Product Information

Product Name

Anti-PCNA Antibody Picoband® (monoclonal, 2G2)
View all PCNA Antibodies

Form

Lyophilized

Uniprot

P12004

Gene ID

5111

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

2G2

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human PCNA recombinant protein (Position: M1-S261).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

36 kDa

Calculated Molecular Weight

28.8 kDa

Background of PCNA

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Cite This Product

Anti-PCNA Antibody Picoband® (monoclonal, 2G2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00125-3)

Protocols

Western Blot protocol for PCNA antibody

Review Protocol

Immunohistochemistry (IHC) protocol for PCNA antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PCNA antibody

Review Protocol

Flow Cytometry (FC) protocol for PCNA antibody

Review Protocol

Publications

Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells

Published: 2025-11-24

Journal: Biology-Basel

Application: IF

Impact Factor: 3.50

Species: Mouse

Bundle Save

Bundle & Save Program

BUY MORE, SAVE MORE

Free Secondary Antibody

Anti-PCNA Antibody Picoband® (monoclonal, 2G2)

PCNA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells

Tailoring Carrier-Free Nanoparticles based on Natural Small Molecule Assembly for Synergistic Anti-tumor Efficacy

Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis

Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes

DNA methylation-mediated repression of microRNA-410 promotes the growth of human glioma cells and triggers cell apoptosis through its interaction with STAT3

Autophagy-related 7 protein-dependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell

CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis

Simultaneous Inhibition of Ornithine Decarboxylase 1 and Pyruvate Kinase M2 Exerts Synergistic Effects Against Hepatocellular Carcinoma Cells

Interaction between DMRT1 and PLZF protein regulates self-renewal and proliferation in male germline stem cells

Interferon regulatory factor 7 inhibits rat vascular smooth muscle cell proliferation and inflammation in monocrotaline-induced pulmonary hypertension

Postnatal growth retardation is associated with deteriorated intestinal mucosal barrier function using a porcine model

Nanocrystallized Oleanolic Acid Better Inhibits Proliferation, Migration and Invasion in Intracranial Glioma via Caspase-3 Pathway

Therapeutic sildenafil inhibits pulmonary damage induced by cigarette smoke exposure and bacterial inhalation in rats

Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D

Hypoxia promotes pulmonary vascular remodeling via HIF-1α to regulate mitochondrial dynamics

Isoprenaline protects intestinal stem cells from chemotherapy-induced damage

SETD3 is regulated by a couple of microRNAs and plays opposing roles in proliferation and metastasis of hepatocellular carcinoma

Zn(II)-curcumin solid dispersion impairs hepatocellular carcinoma growth and enhances chemotherapy by modulating gut microbiota-mediated zinc homeostasis

Enhanced cytotoxic T lymphocytes recruitment targeting tumor vasculatures by endoglin aptamer and IP-10 plasmid presenting liposome-based nanocarriers

Human Amniotic Epithelial Cell-Derived Exosomes Restore Ovarian Function by Transferring MicroRNAs against Apoptosis

Long noncoding RNA Hoxaas3 contributes to hypoxia-induced pulmonary artery smooth muscle cell proliferation

IL-33 facilitates proliferation of colorectal cancer dependent on COX2/PGE2

Caveolin‑1 facilitated KCNA5 expression, promoting breast cancer viability

The protective effects of Aquilariae Lignum Resinatum extract on 5-Fuorouracil-induced intestinal mucositis in mice

Lentivirus-mediated down-regulation of CK2α inhibits proliferation and induces apoptosis of malignant lymphoma and leukemia cells

The enhanced antitumor-specific immune response with mannose- and CpG-ODN-coated liposomes delivering TRP2 peptide

Self-Assembled Polyprodrug Amphiphile for Subcutaneous Xenograft Tumor Inhibition with Prolonged Acting Time In Vivo

High Expression of Pirh2 is Associated with Poor Prognosis in Glioma

Resveratrol Enhances Self-Renewal of Mouse Embryonic Stem Cells

Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head

Reparative activity of costunolide and dehydrocostus in a mouse model of 5-fluorouracil-induced intestinal mucositis

NLRC5 Mediates IL-6 and IL-1β Secretion in LX-2 Cells and Modulated by the NF-κB/Smad3 Pathway

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

The cell death and DNA damages caused by the Tet-On regulating HSV-tk/GCV suicide gene system in MCF-7 cells

Dimethylarsinic acid (DMAV) changed the expressions of proliferative related factors and secretion of inflammatory cytokines in rat bladder

Pathway of Programmed Cell Death and Oxidative Stress Induced by β-Hydroxybutyrate in Dairy Cow Abomasum Smooth Muscle Cells and in Mouse Gastric Smooth Muscle

Abscopal antitumor immune effects of magnet‑mediated hyperthermia at a high therapeutic temperature on Walker‑256 carcinosarcomas in rats

Effect of nimesulide on the growth of human laryngeal squamous cell carcinoma

Effects of sterigmatocystin on esophageal epithelium and experimental reflux esophagitis in rats

Oroxylin A Accelerates Liver Regeneration in CCI4-Induced Acute Liver Injury Mice

The Effects of High-Dose Qinggan Huoxue Recipe on Acute Liver Failure Induced by D-Galactosamine in Rats

Downregulation of Id1 by small interfering RNA in prostate cancer PC3 cells in vivo and in vitro

Tumstatin45-132-TNFα suppresses tumour growth through anti-angiogenic effects and cytotoxicity

Absence of the Adenosine A2A Receptor Confers Pulmonary Arterial Hypertension and Increased Pulmonary Vascular Remodeling in Mice

Synthetic radiation-inducible promoters mediated HSV-TK/GCV gene therapy in the treatment of oral squamous cell carcinoma

Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue?

Inhibitory effect of Kangjia Pill (抗甲丸) on thyrocyte proliferation in rat goiter model

Increased expression of focal adhesion kinase correlates with cellular proliferation and apoptosis during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis

Differential expression of the genes involved in amino acids and nitrogen metabolisms during liver regeneration of mice

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

Mesenchymal stem cells derived from bone marrow favor tumor cell growth in vivo

Review

Have you used Anti-PCNA Antibody Picoband® (monoclonal, 2G2)?

Product Promise

Troubleshooting

Boster Guarantee

Guaranteed product quality