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Product Info Summary
| SKU: | M05304 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PDE8A Purified Monoclonal Antibody
SKU/Catalog Number
M05304
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PDE8A Purified Monoclonal Antibody (Catalog# M05304). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-PDE8A Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05304)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
EM-52
Isotype
Mouse IgG1 kappa
Immunogen
Human PDE8a. The mouse monoclonal antibody EM-52 recognizes PDE8a (phosphodiesterase 8 A), an approximately 93 kDa intracellular enzyme that hydrolyzes cAMP to 5'AMP.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M05304 is reactive to PDE8A in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
93.3 kDa
Background of PDE8A
PDE8a (high affinity cAMP-specific and IBMX-insensitive 3´,5´-cyclic phosphodiesterase 8 A) is a cyclic nucleotide phosphodiesterase, which hydrolyzes cAMP, an important regulator and mediator of multiple cellular responses to extracellular signals. It is expressed in a wide variety of tissues including testis, ovary, small intestine, and colon. It is not expressed e.g. in thymus. PDE8a is composed of C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05304 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Western bloting analysis of human PDE8A using mouse monoclonal antibody EM-52 on lysates of HeLa cell line and TF-1 cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-PDE8A followed by Alexa Fluor® 790-conjugated goat-anti-mouse secondary antibody. A specific band was detected for PDE8A at approximately 95 kDa.
Specific Publications For Anti-PDE8A Purified Monoclonal Antibody (M05304)
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