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Product Info Summary
| SKU: | M00096-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-PDGF Receptor beta (PDGFRB) Mouse Monoclonal Antibody [Clone ID: OTI1D2]
SKU/Catalog Number
M00096-3
Size
100 µl
Description
Boster Bio PDGFRB mouse monoclonal antibody, clone OTI1D2 (formerly 1D2). Catalog# M00096-3. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PDGF Receptor beta (PDGFRB) Mouse Monoclonal Antibody [Clone ID: OTI1D2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00096-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1D2
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human PDGFRB (NP_002600) produced in HEK293T cell.
Reactive Species
M00096-3 is reactive to PDGFRB in Human, Mouse, Rat
Calculated molecular weight
124.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00096-3 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IF 1:100
Flow Cytometry 1:50
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDGFRB.
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Flow cytometric Analysis of living SF295 cells
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Anti-PDGFRB mouse monoclonal antibody (M00096-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDGFRB.
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Immunofluorescent staining of HeLa cells using anti-PDGFRB mouse monoclonal antibody (M00096-3).
Specific Publications For Anti-PDGF Receptor beta (PDGFRB) Mouse Monoclonal Antibody [Clone ID: OTI1D2] (M00096-3)
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