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Product Info Summary
| SKU: | A00176-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MAPK14 Antibody (Y323)
SKU/Catalog Number
A00176-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-MAPK14 Antibody (Y323) (Catalog # A00176-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAPK14 Antibody (Y323) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00176-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from human MAPK14.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00176-1 is reactive to MAPK14 in Human, Mouse, Rat
Calculated molecular weight
41.3 kDa
Background of MAPK14
MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00176-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MAPK14 Antibody (Y323) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: PC-12 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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MAPK14 Antibody (Y322) (Cat. #A00176-1) western blot analysis in Jurkat cell line and mouse kidney tissue lysates (35ug/lane). This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).
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All lanes : Anti-MAPK14 Antibody (Y323) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: PC-12 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: MCF-7 whole cell lysate
Lane 6: mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 41 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Overlay histogram showing Hela cells stained with A00176-1 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A00176-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Specific Publications For Anti-MAPK14 Antibody (Y323) (A00176-1)
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