Click image to see more details
Product Info Summary
| SKU: | A01906-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-PDHA1/Pdh E1Alpha Antibody
SKU/Catalog Number
A01906-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PDHA1/Pdh E1Alpha Antibody catalog # A01906-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PDHA1/Pdh E1Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01906-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PDHA1. AA range:314-363
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01906-1 is reactive to PDHA1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01906-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western blot analysis of various cells using PDHA1 Polyclonal Antibody diluted at 1：2000
Specific Publications For Anti-PDHA1/Pdh E1Alpha Antibody (A01906-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-PDHA1/Pdh E1Alpha Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus using anti-PDHA1/Pdh E1Alpha antibody A01906-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-15
Question
I see that the anti-PDHA1/Pdh E1Alpha antibody A01906-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-26
Question
Is this A01906-1 anti-PDHA1/Pdh E1Alpha antibody reactive to the isotypes of PDHA1?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
The immunogen of A01906-1 anti-PDHA1/Pdh E1Alpha antibody is Synthesized peptide derived from the C-terminal region of human PDHA1. at AA rangle: 290-370. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-31
Question
Will A01906-1 anti-PDHA1/Pdh E1Alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
You can see on the product datasheet, A01906-1 anti-PDHA1/Pdh E1Alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-05
Question
Will anti-PDHA1/Pdh E1Alpha antibody A01906-1 work on zebrafish WB with cervix carcinoma erythroleukemia?
K. Miller
Verified customer
Asked: 2014-03-27
Answer
Our lab technicians have not validated anti-PDHA1/Pdh E1Alpha antibody A01906-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PDHA1/Pdh E1Alpha antibody A01906-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-27
