Anti-PDHA1/Pdh E1Alpha Antibody

Boster Bio Anti-PDHA1/Pdh E1Alpha Antibody catalog # A01906-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01906-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-PDHA1/Pdh E1Alpha Antibody

See all Pyruvate Dehydrogenase E1-alpha subunit products

SKU/Catalog Number

A01906-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PDHA1/Pdh E1Alpha Antibody catalog # A01906-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PDHA1/Pdh E1Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01906-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human PDHA1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01906-1 is reactive to PDHA1 in Human, Mouse, Rat

Applications

A01906-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

Gene/Protein Information For PDHA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08559

Gene ID

5160

Gene Name

PDHA1

Full Name

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Weight

43.296kDa

Alternative Names

EC 1.2.4.1; PDHA; PDHE1-A type I; PHE1APDHCE1A; pyruvate dehydrogenase (lipoamide) alpha 1; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial PDHA1 PDHA, PDHAD, PDHCE1A, PHE1A pyruvate dehydrogenase E1 subunit alpha 1 pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial|PDHE1-A type I|pyruvate dehydrogenase (lipoamide) alpha 1|pyruvate dehydrogenase E1 alpha 1 subunit|pyruvate dehydrogenase alpha 1|pyruvate dehydrogenase complex, E1-alpha polypeptide 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PDHA1, check out the PDHA1 Infographic

PDHA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PDHA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-PDHA1/Pdh E1Alpha Antibody

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus using anti-PDHA1/Pdh E1Alpha antibody A01906-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-15

Question

I see that the anti-PDHA1/Pdh E1Alpha antibody A01906-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-26

Question

Is this A01906-1 anti-PDHA1/Pdh E1Alpha antibody reactive to the isotypes of PDHA1?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

The immunogen of A01906-1 anti-PDHA1/Pdh E1Alpha antibody is Synthesized peptide derived from the C-terminal region of human PDHA1. at AA rangle: 290-370. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-31

Question

Will A01906-1 anti-PDHA1/Pdh E1Alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-07-05

Answer

You can see on the product datasheet, A01906-1 anti-PDHA1/Pdh E1Alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-05

Question

Will anti-PDHA1/Pdh E1Alpha antibody A01906-1 work on zebrafish WB with cervix carcinoma erythroleukemia?

K. Miller

Verified customer

Asked: 2014-03-27

Answer

Our lab technicians have not validated anti-PDHA1/Pdh E1Alpha antibody A01906-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PDHA1/Pdh E1Alpha antibody A01906-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-03-27

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A01906-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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