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Product Info Summary
| SKU: | A04110 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PHLPP2 Antibody
SKU/Catalog Number
A04110
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PHLPP2 Antibody (Catalog # A04110). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
PHLPP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PHLPP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04110)
Host
Rabbit
Contents
PHLPP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PHLPP2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2. The immunogen is located within the last 50 amino acids of PHLPP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms are known to exist; this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1.
Reactive Species
A04110 is reactive to PHLPP2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
146751 MW
Background of PHLPP2
PHLPP2 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. PHLPP2 associates with and is inhibited by adenylyl cyclase type 6 (AC6), thereby allowing Akt activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04110 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PHLPP2 antibody can be used for detection of PHLPP2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
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Immunocytochemistry of PHLPP2 in SW480 cells with PHLPP2 antibody at 2.5 μg/ml.
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Immunofluorescence of PHLPP2 in SW480 cells with PHLPP2 antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For PHLPP2 (Source: Uniprot.org, NCBI)
Gene Name
PHLPP2
Full Name
PH domain leucine-rich repeat-containing protein phosphatase 2
Weight
146751 MW
Alternative Names
PHLPPL, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like PHLPP2 PHLPPL, PPM3B PH domain and leucine rich repeat protein phosphatase 2 PH domain leucine-rich repeat-containing protein phosphatase 2|protein phosphatase, Mg2+/Mn2+ dependent 3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PHLPP2, check out the PHLPP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PHLPP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PHLPP2 Antibody (A04110)
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