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Product Info Summary
| SKU: | P00026-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00026-1
BM3952 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody catalog # P00026-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOO-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-c-Myc (T58 + S62)
Reactive Species
P00026-1 is reactive to MYC in Human, Rat
Observed Molecular Weight
57 kDa
Calculated molecular weight
48.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00026-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
FC 1:50
Positive Control
WB: human 293T whole cell
Validation Images & Assay Conditions
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Western blot analysis of P-MYC using anti-P-MYC antibody (P00026-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-MYC antigen affinity purified monoclonal antibody (P00026-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-MYC at approximately 57 kDa. The expected band size for P-MYC is at 49 kDa.
Specific Publications For Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody (P00026-1)
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4 Customer Q&As for Anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal Antibody
Question
We purchased anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody for WB on promyelocytic leukemia a few years ago. I am using rat, and We are going to use the antibody for IP next. I was wanting to use examining promyelocytic leukemia as well as cervix in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I have checked the website and datasheets of our anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody and it seems that P00026-1 has been tested on rat in both WB and IP. Thus P00026-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-01
Question
We are currently using anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody P00026-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody (P00026-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
We have been able to see staining in rat cervix. Are there any suggestions? Is anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody supposed to stain cervix positively?
L. Zhao
Verified customer
Asked: 2018-09-21
Answer
From what I have seen in literature cervix does express MYC. From what I have seen in Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-09-21
Question
Our team were well pleased with the WB result of your anti-Phospho-c-Myc (T58 + S62) Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta testis nucleoplasm using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
M. Edwards
Verified customer
Asked: 2015-09-30
Answer
Based on literature, placenta testis does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-09-30
