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Product Info Summary
| SKU: | P07956-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Phospho-Dok-2 (Y299) Antibody
SKU/Catalog Number
P07956-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-Dok-2 (Y299) Antibody catalog # P07956-1. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Dok-2 (Y299) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07956-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human p56 Dok-2 around the phosphorylation site of Tyr299. AA range:266-315
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P07956-1 is reactive to DOK2 in Human, Monkey, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
45.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P07956-1 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p56 Dok-2 (Phospho-Tyr299) Antibody
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Western Blot analysis of various cells using Phospho-Dok-2 (Y299) Polyclonal Antibody
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Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', Jurkat cells treated with insulin 0.01U/ml 15' and 293 cells treated with serum 20% 15', using p56 Dok-2 (Phospho-Tyr299) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-Dok-2 (Y299) Antibody (P07956-1)
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Customer Reviews
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4 Customer Q&As for Anti-Phospho-Dok-2 (Y299) Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Phospho-Dok-2 (Y299) antibody P07956-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
Would anti-Phospho-Dok-2 (Y299) antibody P07956-1 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
According to the expression profile of leukemic t-cell, DOK2 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho-Dok-2 (Y299) antibody P07956-1 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-08-05
Question
Will P07956-1 anti-Phospho-Dok-2 (Y299) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Parker
Verified customer
Asked: 2019-01-29
Answer
It shows on the product datasheet, P07956-1 anti-Phospho-Dok-2 (Y299) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-29
Question
We are currently using anti-Phospho-Dok-2 (Y299) antibody P07956-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it possible that the antibody can work on pig tissues as well?
P. Brown
Verified customer
Asked: 2017-02-01
Answer
The anti-Phospho-Dok-2 (Y299) antibody (P07956-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-01
