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Product Info Summary
| SKU: | PA1347 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SFTPA1 Antibody Picoband®
SKU/Catalog Number
PA1347
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFTPA1 Antibody catalog # PA1347. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFTPA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1347)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPA1, different from the rat sequence by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1347 is reactive to SFTPA1 in Human, Rat
Observed Molecular Weight
26-35 kDa
Calculated molecular weight
26.2 kDa
Background of SFTPA1
Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14.1. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1347 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1347).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35KD. The expected band size for SFTPA1 is at 26KD.
Specific Publications For Anti-SFTPA1 Antibody Picoband® (PA1347)
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Customer Q&As
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6 Customer Q&As for Anti-SFTPA1 Antibody Picoband®
Question
Will anti-SFTPA1 antibody PA1347 work on goat WB with lung?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Our lab technicians have not validated anti-SFTPA1 antibody PA1347 on goat. You can run a BLAST between goat and the immunogen sequence of anti-SFTPA1 antibody PA1347 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-06
Question
See below the WB image, lot number and protocol we used for right lung using anti-SFTPA1 antibody PA1347. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-25
Question
I see that the anti-SFTPA1 antibody PA1347 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-12
Question
Is there a BSA free version of anti-SFTPA1 antibody PA1347 available?
R. Li
Verified customer
Asked: 2019-02-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SFTPA1 antibody PA1347 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-02-22
Question
Is a blocking peptide available for product anti-SFTPA1 antibody (PA1347)?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We do provide the blocking peptide for product anti-SFTPA1 antibody (PA1347). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-10-11
Question
I have a question about product PA1347, anti-SFTPA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1347 anti-SFTPA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-01
