Rabbit IgG polyclonal antibody for Bcl-2-like protein 1(BCL2L1) detection. Tested with WB, IHC-P in Human;Mouse;Rat. Cited in 5 publication(s).
Anti-Bcl-XS/BCL2L1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Bcl-XS/BCL2L1 Antibody
See all BCL2L1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for BCL XL/BCL2L1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. BCL XL/BCL2L1 information: Molecular Weight: 26049 MW; Subcellular Localization: Isoform Bcl-X(L): Mitochondrion inner membrane . Mitochondrion outer membrane . Mitochondrion matrix . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane ; Single-pass membrane protein ; Cytoplasmic side . After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49; Tissue Specificity: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.|
|Cite This Product||Anti-Bcl-XS/BCL2L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1232)|
|Similar Products From
|Anti-Bcl-XS/BCL2L1 Antibody may replace the following items: sc 13284|sc 13283|sc 25379|sc 398346.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Bcl-XS(120-130aa YQSFEQDTFVE), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For BCL2L1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||598|
|Species Of This Entry||Human|
|Protein Name||Bcl-2-like protein 1|
|Alternative Names||BCL2L1|Apoptosis regulator Bcl-X; bcl2-L-1; BCL2-like 1; bcl-2-like protein 1; BCLXBCL2LBcl-X; BclxL; Bcl-xL; BCL-XL/S; BCLXS; bcl-xS; DKFZp781P2092|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Dephosphorylation; Phosphorothioation; Reduction; Deamidation; Ubiquitination; Acetylation; Methylation; Oxidation; Glycosylation; Myristoylation; Prenylation; Nitrosylation; Hydroxylation; Biotinylation; Sumoylation; Demethylation; Amidation; Deglutathionylation|
|Subcellular Localization||[Isoform Bcl-X(L)]: Mitochondrion inner membrane. Mitochondrion outer membrane. Mitochondrion matrix. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side. After neuronal stimulation, translocates from cytosol to synaptic vesicle and mitochondrion membrane in a calmodulin-dependent manner (By similarity). Localizes to the centrosome when phosphorylated at Ser-49.|
|Gene Location||20q11.21, on Chromasome 20, gene sequence: NC_000020.11 (31664452..31723963, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For BCL2L1 (Source: Uniprot.org, NCBI)
|Protein Function||Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage- dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.|
|Tissue Specificity||Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.|
|Interacting Proteins||BAD; Bad; BAK1; BAX; BBC3; Bbc3; BCL2L11; BECN1; BID; BIK; BMF; CYCS; Gimap3; Gimap5; HRK; MTOR; NLRP1; RTL10; SIVA1; SPNS1; TP53; Tp53; TP53BP2|
|Research Areas||Apoptosis; Cancer; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Leukemia; Malignant Paraganglionic Neoplasm; Lymphoma; Mammary Neoplasms; Cell Transformation, Neoplastic; Malignant Neoplasm Of Breast; Myeloid Leukemia; Adenocarcinoma; Prostatic Neoplasms; Malignant Neoplasm Of Prostate; Ischemia; Liver Carcinoma; Nervousness; Inflammation; Malignant Tumor Of Colon; Neoplasm Metastasis; Colonic Neoplasms|
|Related Pathways||Cell Death; Cell Cycle; Induction Of Apoptosis; Programmed Cell Death; Cell Proliferation; Cell Growth; Pathogenesis; Localization; Cell Cycle Arrest; Drug Resistance; Apoptotic Process; Secretion; Sensitization; Rna Interference; Cell Activation; Regeneration; Immune Response; Cell Differentiation; Release Of Cytochrome C From Mitochondria; Transport|
|Background||Bcl-xS is the second mRNA species of Bcl-x protein, which encodes a protein that inhibits the ability of bcl-2 to enhance the survival of growth factor-deprived cells. In vivo, bcl-xS mRNA is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Apoptosis was associated with increased protein expression of Bcl-X/S, an early marker of apoptosis, and release of cytochrome c into the cytosol of infected HB cells.|
|Scientific References||PMID: 8358789 by Boise L.H., et al. bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death.|
PMID: 9675101 by Ban J., et al. Identification of a human cDNA encoding a novel Bcl-x isoform.
Our Boster Quality Guarantee for Anti-Bcl-XS/BCL2L1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Bcl-XS (PA1232) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Predicted bind size: 19KD
Observed bind size: 19KD
IHC(P): Human Colon Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Zhou X, Zhang Y, Li Y, Hao X, Liu X, Wang Y. Cancers (Basel). 2012 Dec 4;4(4):1318-32. Doi: 10.3390/Cancers4041318. Azithromycin Synergistically Enhances Anti-Proliferative Activity Of Vincristine In Cervical And Gastric Cancer Cells.||PubMed ID 24213508|
|Emodin alleviates severe acute pancreatitis-associated acute lung injury by decreasing pre-B-cell colony-enhancing factor expression and promoting polymorphonuclear neutrophil apoptosis||PubMed ID 28849044|
|Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions||PubMed ID 12439908|
|Myricetin exhibits anti-glioma potential by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and ROS generation||PubMed ID 27061547|
|Reactive oxygen species-induced apoptosis in PC12 cells and protective effect of bilobalide||PubMed ID 10869401|
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Note: sample size takes longer to process, average 5-7 days.
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