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Product Info Summary
| SKU: | PA1232 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bcl-XS/BCL2L1 Antibody Picoband®
SKU/Catalog Number
PA1232
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bcl-XS/BCL2L1 Antibody catalog # PA1232. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-XS/BCL2L1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1232)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Bcl-XS, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1232 is reactive to BCL2L1 in Human, Mouse, Rat
Observed Molecular Weight
18-20 kDa
Calculated molecular weight
26.0 kDa
Background of BCL2L1
Bcl-xS is the second mRNA species of Bcl-x protein, which encodes a protein that inhibits the ability of bcl-2 to enhance the survival of growth factor-deprived cells. In vivo, bcl-xS mRNA is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Apoptosis was associated with increased protein expression of Bcl-X/S, an early marker of apoptosis, and release of cytochrome c into the cytosol of infected HB cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1232 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human U87 whole cell, rat pancrease tissue, mouse pancrease tissue
IHC: Human Colon Cancer Tissue
Validation Images & Assay Conditions
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Western blot analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XS/BCL2L1 antigen affinity purified polyclonal antibody (PA1232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Bcl-XS/BCL2L1 at approximately 18-20 kDa. The expected band size for Bcl-XS/BCL2L1 is at 18 kDa.
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Western blot analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancrease tissue lysates,
Lane 2: mouse pancrease tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XS/BCL2L1 antigen affinity purified polyclonal antibody (PA1232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Bcl-XS/BCL2L1 at approximately 18-20 kDa. The expected band size for Bcl-XS/BCL2L1 is at 18 kDa.
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Anti-Bcl-XS antibody, PA1232, IHC(P)
IHC(P): Human Colon Cancer Tissue
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IHC analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232).
Bcl-XS/BCL2L1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bcl-XS/BCL2L1 Antibody (PA1232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Bcl-XS/BCL2L1 Antibody Picoband® (PA1232)
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17 Customer Q&As for Anti-Bcl-XS/BCL2L1 Antibody Picoband®
Question
My question regarding product PA1232, anti-Bcl-XS/BCL2L1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1232 anti-Bcl-XS/BCL2L1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-27
Question
I see that the anti-Bcl-XS/BCL2L1 antibody PA1232 works with IHC, what is the protocol used to produce the result images on the product page?
C. Zhao
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-24
Question
I was wanting to use your anti-Bcl-XS/BCL2L1 antibody for IHC for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, PA1232 anti-Bcl-XS/BCL2L1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Bcl-XS/BCL2L1 antibody PA1232. Let me know if you need anything else.
A. Carter
Verified customer
Asked: 2019-09-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-26
Question
Our team were content with the WB result of your anti-Bcl-XS/BCL2L1 antibody. However we have seen positive staining in colon carcinoma isoform bcl-x(l): mitochondrion inner using this antibody. Is that expected? Could you tell me where is BCL2L1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Based on literature, colon carcinoma does express BCL2L1. Generally BCL2L1 expresses in isoform bcl-x(l): mitochondrion inner. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-Bcl-XS/BCL2L1 antibody PA1232 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-Bcl-XS/BCL2L1 antibody (PA1232) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
Question
See below the WB image, lot number and protocol we used for lung using anti-Bcl-XS/BCL2L1 antibody PA1232. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Will anti-Bcl-XS/BCL2L1 antibody PA1232 work for IHC with lung?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
According to the expression profile of lung, BCL2L1 is highly expressed in lung. So, it is likely that anti-Bcl-XS/BCL2L1 antibody PA1232 will work for IHC with lung.
Boster Scientific Support
Answered: 2019-06-06
Question
My lab would like to test anti-Bcl-XS/BCL2L1 antibody PA1232 on mouse lung for research purposes, then I may be interested in using anti-Bcl-XS/BCL2L1 antibody PA1232 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The products we sell, including anti-Bcl-XS/BCL2L1 antibody PA1232, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-24
Question
Is this PA1232 anti-Bcl-XS/BCL2L1 antibody reactive to the isotypes of BCL2L1?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The immunogen of PA1232 anti-Bcl-XS/BCL2L1 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Bcl-XS(120-130aa YQSFEQDTFVE), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-15
Question
We have observed staining in human upper lobe of left lung. Do you have any suggestions? Is anti-Bcl-XS/BCL2L1 antibody supposed to stain upper lobe of left lung positively?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
Based on literature upper lobe of left lung does express BCL2L1. Based on Uniprot.org, BCL2L1 is expressed in upper lobe of left lung, colon carcinoma, lung, among other tissues. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-21
Question
Is a blocking peptide available for product anti-Bcl-XS/BCL2L1 antibody (PA1232)?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
We do provide the blocking peptide for product anti-Bcl-XS/BCL2L1 antibody (PA1232). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-01
Question
We have tried in the past anti-Bcl-XS/BCL2L1 antibody for WB on colon carcinoma in a previous project. I am using mouse, and I plan to use the antibody for IHC next. I would like examining colon carcinoma as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
I looked at the website and datasheets of our anti-Bcl-XS/BCL2L1 antibody and I see that PA1232 has been tested on mouse in both WB and IHC. Thus PA1232 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-23
Question
Will PA1232 anti-Bcl-XS/BCL2L1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-12
Answer
As indicated on the product datasheet, PA1232 anti-Bcl-XS/BCL2L1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-12
Question
Will anti-Bcl-XS/BCL2L1 antibody PA1232 work on zebrafish WB with colon carcinoma?
B. Banerjee
Verified customer
Asked: 2015-09-17
Answer
Our lab technicians have not validated anti-Bcl-XS/BCL2L1 antibody PA1232 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Bcl-XS/BCL2L1 antibody PA1232 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish colon carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-17
Question
I am interested in using your anti-Bcl-XS/BCL2L1 antibody for the nlrp1 inflammasome studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
E. Li
Verified customer
Asked: 2014-09-25
Answer
We appreciate your inquiry. This PA1232 anti-Bcl-XS/BCL2L1 antibody is validated on hela whole cell lysate, colon cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-09-25
Question
Do you have a BSA free version of anti-Bcl-XS/BCL2L1 antibody PA1232 available?
G. Anderson
Verified customer
Asked: 2013-06-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-XS/BCL2L1 antibody PA1232 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-06-13
