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Product Info Summary
| SKU: | A06717T17 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody
SKU/Catalog Number
A06717T17
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody catalog # A06717T17. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06717T17)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1 around the phosphorylation site of Thr17. AA range:1-50
Reactive Species
A06717T17 is reactive to HIST1H1B in Human, Monkey, Mouse
Calculated molecular weight
22.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06717T17 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of SKOV3 using p-Histone H1 (T17) antibody. Antibody was diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from Jurkat cells treated with UV 15', using Histone H1 (Phospho-Thr17) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Histone H1 (Phospho-Thr17) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of Hela cell. 1, Histone H1 (phospho Thr17) Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). NSE Monoclonal Antibody (13E2) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H1 (Phospho-Thr17) Antibody
Specific Publications For Anti-Phospho-Histone H1 (T17) HIST1H1B Antibody (A06717T17)
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