Product Info Summary
SKU: | A06714 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NF-Y NFYB Antibody
SKU/Catalog Number
A06714
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-NF-Y NFYB Antibody (Catalog # A06714). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-NF-Y NFYB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06714)
Host
Rabbit
Contents
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06714 is reactive to NFYB in Human
Applications
A06714 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
22.831kDa
Background of NFYB
Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:25,000
Gel Shift: 2.0 to 4.0 µl
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
NF-Y was detected in paraffin-embedded sections of human thymus tissues using rabbit anti-NF-Y Antigen Affinity purified polyclonal antibody (Catalog # A06714) at 1:500. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For NFYB (Source: Uniprot.org, NCBI)
Gene Name
NFYB
Full Name
Nuclear transcription factor Y subunit beta
Weight
22.831kDa
Superfamily
NFYB/HAP3 subunit family
Alternative Names
CAAT box DNA-binding protein subunit B; CBF-A; HAP3CBF-B; NF-YBCCAAT-binding transcription factor subunit A; Nuclear transcription factor Y subunit B; nuclear transcription factor Y subunit beta; nuclear transcription factor Y, beta; Transcription factor NF-Y, B subunit NFYB CBF-A, CBF-B, HAP3, NF-YB nuclear transcription factor Y subunit beta nuclear transcription factor Y subunit beta|CAAT box DNA-binding protein subunit B|CCAAT-binding transcription factor subunit A|Transcription factor NF-Y, B subunit|nuclear transcription factor Y subunit B|nuclear transcription factor Y, beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFYB, check out the NFYB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFYB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-Y NFYB Antibody (A06714)
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No publications found for A06714
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