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- Table of Contents
H2AFX antibody (MP00241) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Immunofluorescent analysis of HeLa cells treated with H2O2, using Phospho-Histone H2A.X (S139) Antibody.
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Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody
H2AFX antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
H2AFX antibody was positively detected by WB in:
human Jurkat whole cell, human U20S whole cell, human PC-3 whole cell, human 293T whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
H2AFX antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human liver cancer tissue, human placenta tissue, mouse liver tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
H2AFX antibody was positively detected by ICC/IF in:
HeLa cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody
View all H2AFX Antibodies
View all H2AFX Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AbH41
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-Histone H2A.X (S139)
Observed Molecular Weight
15 kDa
Calculated Molecular Weight
15.1 kDa
Cite This Product
Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MP00241)
Protocols
Western Blot protocol for H2AFX antibody
Immunohistochemistry (IHC) protocol for H2AFX antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for H2AFX antibody
Publications
Application:
Species:
PDHA1 Hyperactivation Orchestrates Metabolic Reprogramming Promoting Endothelial Senescence
Published: 2026-08-20
Journal: Research
Application: WB
Impact Factor: 12.90
Species: Mouse
PMID: 42625807
Structural Modification and Prodrug Design Based on Natural Stilbene Scaffold for the Discovery of Novel PARP-1 Inhibitors with Improved Antitumor Activity
Published: 2026-07-20
Journal: JOURNAL OF MEDICINAL CHEMISTRY
Application: IF
Impact Factor: 7.30
Species: Human
PMID: 42475630
FOXP1 deficiency exacerbates cellular senescence in PM2.5-induced renal injury
Published: 2026-03-30
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB,IF
Impact Factor: 6.10
Species: Mouse,Human
PMID: 41916119
Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice
Published: 2026-03-06
Journal: CELLULAR AND MOLECULAR LIFE SCIENCES
Application: IHC
Impact Factor: 6.20
Species: Mouse
PMID: 41792311
Pt(IV)-PROTAC Complexes with Synergistic Antitumor Activity and Enhanced Membrane Permeability
Published: 2025-04-04
Journal: JOURNAL OF MEDICINAL CHEMISTRY
Application: IF
Impact Factor: 6.80
Species: Human
PMID: 40184539
Krüppel-like transcription factor 14 alleviates alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress in pulmonaryfibrosis
Published: 2024-09-11
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 7.70
Species: Mouse
PMID: 39270890
Novel pyridazinone derivatives bind to KSRP: Synthesis, anti-tumor biological evaluations and modelling insights
Published: 2024-08-30
Journal: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Application: IHC
Impact Factor: 6.00
Species: Mouse
PMID: 39217859
Carbamoylation at C-8 position of natural 3-arylcoumarin scaffold for the discovery of novel PARP-1 inhibitors with potent anticancer activity
Published: 2024-07-31
Journal: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
Application: IF
Impact Factor: 6.00
Species: Human
PMID: 39116535
Epoxyeicosatrienoic acids alleviate alveolar epithelial cell senescence by inhibiting mitophagy through NOX4/Nrf2 pathway
Published: 2023-11-25
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 7.50
Species: Mouse
PMID: 38007934
Design of new Zn(II) pyrazine and pyridine derivatives based complexes as potential anticancer agents: from synthesis to structure
Published: 2023-09-03
Journal: POLYHEDRON
Application: ELISA
Impact Factor: 2.60
Species: Human
EETs alleviate alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress through the Trim25/Keap1/Nrf2 axis
Published: 2023-05-28
Journal: Redox Biology
Application: WB
Impact Factor: 11.40
Species: Mouse
PMID: 37269686
Transcriptome Sequencing Reveals Tgf-β-Mediated Noncoding RNA Regulatory Mechanisms Involved in DNA Damage in the 661W Photoreceptor Cell Line
Published: 2022-11-17
Journal: Genes
Application: IF
Impact Factor: 4.14
Species: Mouse
PMID: 36421815
COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR
Published: 2022-07-27
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 6.21
Species: Mouse
PMID: 35897843
Review
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Product Promise
We promise Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
MP00241
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026