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Product Info Summary
| SKU: | M00358 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDK6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00358
BM4671 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDK6 Rabbit Monoclonal Antibody catalog # M00358. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00358)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HOA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDK6
Reactive Species
M00358 is reactive to CDK6 in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
36.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00358 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human Hela whole cell, human 293T whole cell, human THP-1 whole cell, human Jurkat whole cell
IHC: human lung cancer tissue
ICC/IF: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of CDK6 using anti-CDK6 antibody (M00358).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK6 antigen affinity purified monoclonal antibody (Catalog # M00358) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK6 at approximately 37 kDa. The expected band size for CDK6 is at 37 kDa.
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Immunohistochemical analysis of paraffin-embedded human lung cancer, using CDK6 Antibody.
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Immunofluorescent analysis of K562 cells, using CDK6 Antibody.
Specific Publications For Anti-CDK6 Rabbit Monoclonal Antibody (M00358)
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Customer Q&As
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16 Customer Q&As for Anti-CDK6 Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-CDK6 Rabbit Monoclonal antibody M00358 available?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CDK6 Rabbit Monoclonal antibody M00358 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this M00358 anti-CDK6 Rabbit Monoclonal antibody reactive to the isotypes of CDK6?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of M00358 anti-CDK6 Rabbit Monoclonal antibody is A synthesized peptide derived from human CDK6 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
I have a question about product M00358, anti-CDK6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00358 anti-CDK6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-02
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 Rabbit Monoclonal antibody M00358. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-21
Question
Is a blocking peptide available for product anti-CDK6 Rabbit Monoclonal antibody (M00358)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We do provide the blocking peptide for product anti-CDK6 Rabbit Monoclonal antibody (M00358). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-09
Question
I was wanting to use using your anti-CDK6 Rabbit Monoclonal antibody for primary studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I appreciate your inquiry. This M00358 anti-CDK6 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-03
Question
Our team were satisfied with the WB result of your anti-CDK6 Rabbit Monoclonal antibody. However we have seen positive staining in leukemic t-cell cytoplasm. nucleus. cell projection using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
From what I have seen in literature, leukemic t-cell does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-13
Question
We have tried in the past anti-CDK6 Rabbit Monoclonal antibody for IF on tongue in a previous experiment. I am using human, and We want to use the antibody for WB next. My question regards examining tongue as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
I have checked the website and datasheets of our anti-CDK6 Rabbit Monoclonal antibody and I see that M00358 has been tested on human in both IF and WB. Thus M00358 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-CDK6 Rabbit Monoclonal antibody M00358 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
The anti-CDK6 Rabbit Monoclonal antibody (M00358) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-09
Question
I would like to test anti-CDK6 Rabbit Monoclonal antibody M00358 on human leukemic t-cell for research purposes, then I may be interested in using anti-CDK6 Rabbit Monoclonal antibody M00358 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
The products we sell, including anti-CDK6 Rabbit Monoclonal antibody M00358, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-CDK6 Rabbit Monoclonal antibody for IF for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
It shows on the product datasheet, M00358 anti-CDK6 Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 Rabbit Monoclonal antibody M00358. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-08
Question
Does M00358 anti-CDK6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
As indicated on the product datasheet, M00358 anti-CDK6 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-23
Question
Will anti-CDK6 Rabbit Monoclonal antibody M00358 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
According to the expression profile of leukemic t-cell, CDK6 is highly expressed in leukemic t-cell. So, it is likely that anti-CDK6 Rabbit Monoclonal antibody M00358 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2017-06-13
Question
We have seen staining in human tongue. Do you have any suggestions? Is anti-CDK6 Rabbit Monoclonal antibody supposed to stain tongue positively?
R. Patel
Verified customer
Asked: 2015-08-31
Answer
From literature tongue does express CDK6. From Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-08-31
Question
I see that the anti-CDK6 Rabbit Monoclonal antibody M00358 works with IF, what is the protocol used to produce the result images on the product page?
T. Roberts
Verified customer
Asked: 2015-03-25
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-03-25
