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Product Info Summary
| SKU: | PB9534 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cdk2 Antibody Picoband®
SKU/Catalog Number
PB9534
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cdk2 Antibody Picoband® catalog # PB9534. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdk2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9534)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9534 is reactive to CDK2 in Human
Observed Molecular Weight
30 kDa
Calculated molecular weight
33.9 kDa
Background of CDK2
CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9534 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Jurlat whole cell, human HepG2 whole cell, human U2OS whole cell, human K562 whole cell, human T47D whole cell, human CACO-2 whole cell, human 293T whole cell
IHC: human invasive urothelial carcinoma of the bladder with squamous differentiation tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of Cdk2 using anti-Cdk2 antibody (PB9534).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurlat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human T47D whole cell lysates,
Lane 6: human CACO-2 whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk2 antigen affinity purified polyclonal antibody (Catalog # PB9534) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30, 33 kDa.
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IHC analysis of Cdk2 using anti-Cdk2 antibody (PB9534).
Cdk2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdk2
Antibody (PB9534) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IF analysis of Cdk2 using anti-Cdk2 antibody (PB9534) and anti-Beta Tubulin antibody (M01857-3).
Cdk2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-Cdk2 Antibody (PB9534) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Cdk2 Antibody Picoband® (PB9534)
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5 Customer Q&As for Anti-Cdk2 Antibody Picoband®
Question
My team were satisfied with the WB result of your anti-Cdk2 antibody. However we have been able to see positive staining in placenta cytoskeleton using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Based on literature, placenta does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-09
Question
We have seen staining in mouse cervix carcinoma. Any tips? Is anti-Cdk2 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
According to literature cervix carcinoma does express CDK2. According to Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-24
Question
We ordered your anti-Cdk2 antibody for WB on cervix carcinoma a few years ago. I am using mouse, and We intend to use the antibody for IHC-P next. you antibody examining cervix carcinoma as well as left lobe of thyroid gland in our next experiment. Do you have any suggestion on which antibody would work the best for IHC-P?
C. Jackson
Verified customer
Asked: 2019-02-15
Answer
I viewed the website and datasheets of our anti-Cdk2 antibody and it seems that PB9534 has been validated on mouse in both WB and IHC-P. Thus PB9534 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-15
Question
I was wanting to use using your anti-Cdk2 antibody for regulation of tp53 degradation studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
I appreciate your inquiry. This PB9534 anti-Cdk2 antibody is validated on rat liver tissue, kidney tissue, tissue lysate, human placenta tissue, hela whole cell lysate, hut whole cell lysate, jurkat whole cell lysate, a549 whole cell lysate, mouse testis tissue, lung cancer tissue. It is guaranteed to work for IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-17
Question
We are currently using anti-Cdk2 antibody PB9534 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
The anti-Cdk2 antibody (PB9534) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-19
