This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CDK2 antibody (PB9534) performs well in IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 14 Publications
Filter:
IHC analysis of Cdk2 using anti-Cdk2 antibody (PB9534).
Cdk2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdk2 Antibody (PB9534) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Cdk2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdk2 Antibody (PB9534) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot or IHC for CDK2? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
Bundle Save
Anti-Cdk2 Antibody Picoband®
CDK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IF, IHC, ICC, WB |
Tested Applications
CDK2 antibody was positively detected by WB in:
human Jurlat whole cell, human HepG2 whole cell, human U2OS whole cell, human K562 whole cell, human T47D whole cell, human CACO-2 whole cell, human 293T whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDK2 antibody was positively detected by IHC in:
human invasive urothelial carcinoma of the bladder with squamous differentiation tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CDK2 antibody was positively detected by ICC/IF in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cdk2 Antibody Picoband®
View all CDK2 Antibodies
View all CDK2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
33.9 kDa
Background of CDK2
CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34 (CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34 (cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34 (cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34 (cdc2). P33 (cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Cite This Product
Anti-Cdk2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9534)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
100%
High Homology
Rat
99%
High Homology
Rabbit
99%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Zebrafish
89%
Moderate Homology
Drosophila
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CDK2 antibody
Immunohistochemistry (IHC) protocol for CDK2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CDK2 antibody
Publications
Application:
Species:
Cordycepin inhibits pancreatic cancer progression and enhances gemcitabine sensitivity by targeting the CDK2/E2F2 axis to modulate TNNI2 expression
Published: 2026-07-29
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 6.50
Species: Human
PMID: 42526773
eIF6: a promising therapeutic target for gastric carcinoma via PI3K/AKT pathway modulation
Published: 2025-04-01
Journal: World Journal of Surgical Oncology
Application: WB
Impact Factor: 2.50
PMID: 40170052
5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2
Published: 2025-01-10
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 8.50
Species: Human
PMID: 39800017
ATG10 overexpression is related to the dismal prognosis and promotes the growth and migration of hepatocellular carcinoma cells via cyclin B1/CDK1 and CDK2
Published: 2023-04-15
Journal: American Journal of Cancer Research
Application: WB
Impact Factor: 5.30
Species: Human
PMID: 37168332
IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma
Published: 2018-10-19
Journal: Cancer Communications
Application: WB
Impact Factor: 0.00
Species: Human
PMID: 30340635
Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis
Published: 2018-05-27
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 3.23
Species: Rabbit,Human
PMID: 29847774
OAMDP, a novel podophyllotoxin derivative, induces apoptosis, cell cycle arrest and autophagy in hepatoma HepG2 cells
Published: 2017-10-20
Journal: CELL BIOLOGY INTERNATIONAL
Application: WB
Impact Factor: 1.83
Species: Human
PMID: 29052919
Dehydrocostus Lactone Suppresses Proliferation of Human Chronic Myeloid Leukemia Cells Through Bcr/Abl-JAK/STAT Signaling Pathways
Published: 2017-03-16
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.09
Species: Human
PMID: 28300289
2,3,7,8-Tetrachlorodibenzo-p-dioxin Mediated Cleft palate by Mouse Embryonic Palate Mesenchymal Cells
Published: 2016-08-02
Journal: ARCHIVES OF ORAL BIOLOGY
Application: WB
Impact Factor: 1.73
Species: Mouse
PMID: 27505035
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene
Published: 2016-07-13
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.49
Species: Human
PMID: 27432230
Effects of tripterygium glycosides on restenosis following endovascular treatment
Published: 2016-04-20
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.56
Species: Rabbit
PMID: 27108914
Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins
Published: 2013-09-25
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.41
Species: Human
PMID: 24076388
Anticancer effect of Lycium barbarum polysaccharides on colon cancer cells involves G0/G1 phase arrest
Published: 2010-01-12
Journal: MEDICAL ONCOLOGY
Application: WB
Impact Factor: 1.23
Species: Human
PMID: 20066520
Growth inhibition and cell-cycle arrest of human gastric cancer cells by Lycium barbarum polysaccharide
Published: 2009-08-11
Journal: MEDICAL ONCOLOGY
Application: WB
Impact Factor: 1.13
Species: Human
PMID: 19669955
Review
Have you used Anti-Cdk2 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Cdk2 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9534
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026