Anti-Phospho-IL8 beta (S347) CXCR2 Antibody

Upregulation of the expression of CXCR2 protein in TG in orofacial itch. (A) Double immunohistochemistry of CXCR2 and DAPI at 6 h and day 1 after incision. (B) Statistical analysis of CXCR2 immunofluorescence density at 6 h and day 1 after incision ( n = 3 mice and 8–9 sections for each group). (C) Representative Western blot of CXCR2 in TG with incision. (D) Statistical analysis of CXCR2 protein with incision ( n = 3 mice for each group) (E) Double immunohistochemistry of CXCR2 and IB4 with CQ and histamine injection. (F) Mean number of CXCR2 neurons after CQ and histamine injection ( n = 3 mice and 6–8 sections for each group). Scale bars, 50 μm. One-way ANOVA followed by Dunnett’s multiple comparisons test in (B,D,G,H) . * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SEM.
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Effects of the CXCR2 Antagonist SB225002 on oroficial itch. (A) Double immunohistochemistry of CXCR2 and IB4 in the saline and SB225002 treatment groups. (B) Statistical analysis of the immunofluorescence density of CXCR2 in saline and SB225002 treatment ( n = 3 mice and 11 sections for each group). (C) Representative Western blot of CXCR2 with saline and SB225002 after incision. (D) Statistical analysis of CXCR2 protein with saline or SB225002 after incision ( n = 3 mice for each group). (E) Effects of SB225002 on scratch behaviors induced by incision ( n = 10 mice for each group). (F) Effects of SB225002 on scratch behaviors induced by CQ and histamine ( n = 8 mice for each group). Scale bars, 50 μm. Unpaired t test in (B,D,F) and 2-way ANOVA in (E) . * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SEM.
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DION microinjection and effects of CXCR2 shRNA on orofacial itch. (A) Schematic of DiI and shRNA virus microinjection into DION. (B) Double immunohistochemistry of CXCR2 and DiI in TG neurons ( n = 3 mice and 10 sections). Arrows indicate DiI-labeled CXCR2 neurons. (C) The proportion of DiI-positive neurons in TG neurons ( n = 3 mice and 10 sections for each group). (D) Examples of immunofuorescence images of CXCR2 and GFP treated with scramble and shRNA. (E) Statistical analysis of CXCR2 immunofluorescence density with scramble and shRNA ( n = 3 mice and 9 sections for each group). (F) Representative Western blot of CXCR2 with scramble and shRNA. (G) Statistical analysis of CXCR2 protein in TG with scramble and shRNA ( n = 3 mice for each group). (H) Effects of CXCR2 shRNA injection on incision-induced scratch behaviors ( n = 5–6 mice for each group). (I) Effects of the CXCR2 shRNA virus on scratch behaviors induced by CQ and histamine ( n = 6–7 mice for each group). Scale bars, 50 μm. Unpaired t test in (E,G,I) and 2-way ANOVA in (H) . * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SEM.
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Effects of the CXCR2 and PI3K/Akt signal pathway on orofacial itch. (A) Double immunohistochemistry of CXCR2 and Akt in TG. Arrows indicate CXCR2 and Akt positive neurons. (B) Representative Western blot of p-Akt, Akt, and CXCR2 with saline and LY294002 after incision. (C) Statistical analysis of the p-Akt protein with saline and LY294002 after incision ( n = 3 mice for each group). (D) Statistical analysis of Akt protein with saline and LY294002 after incision ( n = 4 mice for each group). (E) Statistical analysis of the CXCR2 protein with saline and LY294002 after incision ( n = 3 mice for each group). (F) Effects of LY294002 on incision-induced scratch behavior ( n = 5 mice for each group). (G) Effects of LY294002 on incision-induced scratch behaviors ( n = 7 mice for each group). Scale bars, 50 μm. One-way ANOVA followed by Tukey’s multiple comparisons test in (C,D,E) , 2-way ANOVA in (F) , and unpaired t test in (G) . * p < 0.05, ** p < 0.01. Error bars represent the mean ± SEM.
Index in PubMed under a CC BY license. PMID: 37953876