Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine™ Quick ELISA Kit

Mouse CXCL5 / LIX / ENA-78 / GCP 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: FEK0919
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Assay Time: 90 Minutes

Product Name

Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine™ Quick ELISA Kit

See all Cxcl5 products

SKU/Catalog Number

FEK0919

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CXCL5 / LIX / ENA-78 / GCP 2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Mouse Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0919)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V45-A118

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0919 is reactive to Cxcl5 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Application Guarantee

FEK0919 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0919-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0919-STMouse Cxcl5 Standard 2 10 ng/tube
FEK0919-CMouse Cxcl5 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)015.631.262.51252505001000
O.D.0.0050.3630.6391.1001.8002.2242.2262.317

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3516638836150360
Standard deviation2.178.1316.932.488.120.52
CV (%)6.2%4.9%4.7%6.9%5.4%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cxcl5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135343234331.083.2%
Sample 216618016215216510.046%
Sample 338840139045840928.576.9%
*number of samples for each test n=16.

Gene/Protein Information For Cxcl5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50228

Gene ID

20311

Gene Name

Cxcl5

Full Name

C-X-C motif chemokine 5

Weight

14.235kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

LIX Cxcl5|AMCF, AMCF-II, Cxcl, Cxcl6, ENA-, ENA-78, GCP-, GCP-2, L, LIX, Scyb, Scyb5, Scyb6|chemokine (C-X-C motif) ligand 5|C-X-C motif chemokine 5|cytokine LIX|granulocyte chemotactic protein-2|small inducible cytokine B subfamily, member 5|small inducible cytokine subfamily B, member 15|small-inducible cytokine B5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cxcl5, check out the Cxcl5 Infographic

Cxcl5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cxcl5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FEK0919 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Increased neutrophil infiltration, IL-1 production and a SAPHO syndrome-like phenotype in PSTPIP2-deficient mice

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