Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody

Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate.

AKT/mTOR pathway is involved in MOX-induced autophagy of glioma cells. ( A, B ) U251 and C6 cells were incubated with MOX at different concentrations (0, 10, 15, 20 µM) for 48 h, the effects of MOX on the levels of AKT, p-AKT (Ser473), mTOR, p-mTOR (S2448), P70S6K, p-P70S6K (Ser417), GSK3β, p-GSK3β were examined by western blot. Data were presented as the means ± SD of three independent tests. * P < 0.05, ** P < 0.01, *** P < 0.001 as compared with control group. β-actin was used as an internal standard.
Index in PubMed under a CC BY license. PMID: 32913473

Relationship between ciRNA13761/novel-miR-3880/ ELF2 axis and PI3K/AKT/mTOR/S6K1 pathway. (A) Schematic diagram of animal treatment. C57BL/6 mice were injected with novel-miR-3880 or si ELF2 in an interval of three days and four days alternatively. Samples were harvested at day 22. (B) Immunohistochemistry of mammary gland for p-PI3K, p-AKT, p-mTOR and p-S6K1 in Normal Saline, novel-miR-3880 and si ELF2 groups. (C) Protein phosphorylation level of PI3K, AKT, mTOR and S6K1 in mammary gland. (D,E) Effects of novel-miR-3880 and ELF2 on Bcl2/Bax pathway and protein phosphorylation level of PI3K, AKT, mTOR and S6K1 in MEC. (F) PI3K, AKT, mTOR and S6K1 inhibitors suppressed the phosphorylation of PI3K, AKT, mTOR and S6K1 in MEC. (G–J) The role of novel-miR-3880 and si ELF2 in Bcl2/Bax and protein phosphorylation level of PI3K, AKT, mTOR and S6K1 in MEC with PI3K, AKT, mTOR or S6K1 inhibited. (K) Regulation of ciRNA13761 on Bcl2/Bax and PI3K, AKT, mTOR and S6K1 phosphorylation, and the balance effects of novel-miR-3880. (L) Effects of si DOCK1 on Bcl2/Bax and PI3K, AKT, mTOR and S6K1 phosphorylation.
Index in PubMed under a CC BY license. PMID: 32656203

a Western blotting was used to examine mitochondrial apoptotic pathway-related proteins after treatment with control (i), PANI-PEG-CS (ii), Ir(III) complex (iii), and Ir(III)@PANI-PEG-CS (iv). Key proteins involved in apoptosis such as Bax, Bcl-2, cytochrome c, and cleaved caspase-3 were examined to better understand how each treatment affects cell death at the mitochondrial level. b To further investigate the underlying molecular mechanisms, western blotting was used to investigate the PI3K/AKT/mTOR pathway (i), PANI-PEG-CS (ii), Ir(III) complex (iii), and Ir(III)@PANI-PEG-CS (iv). Expression levels of PI3K, AKT (total and phosphorylated), and mTOR were evaluated to assess whether this survival pathway was activated or suppressed. Protein levels were quantified and compared to the control group to determine statistical significance. * p < 0.05, ** p < 0.01, *** p < 0.001 in comparison to the respective control
Index in Springer Nature under a CC BY license. DOI: 10.1186/s12645-025-00336-z

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Phospho-mTOR (S2448) Antibody.

Western blot analysis of Phospho-mTOR (S2448) using anti-Phospho-mTOR (S2448) antibody (P00003).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse 4T1 whole cell lysates,
Lane 2: LPS-stimulated mouse 4T1 whole cell lysates,
Lane 3: Low-dose drug mouse 4T1 whole cell lysates,
Lane 4: Medium-dose drug mouse 4T1 whole cell lysates,
Lane 5: High-dose drug mouse 4T1 whole cell lysates,
Lane 6: Positive control drug mouse 4T1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-mTOR (S2448) antigen affinity purified monoclonal antibody (Catalog # P00003) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for Phospho-mTOR (S2448) at approximately 289 kDa. The expected band size for Phospho-mTOR (S2448) is at 289 kDa.