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Product Info Summary
| SKU: | P00006 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00006
BM4685 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody catalog # P00006. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00006)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HAE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PTEN
Reactive Species
P00006 is reactive to PTEN in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
47.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00006 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Phospho-PTEN (S380) expression in (1) MCF-7 cell treated with alkaline phosphatase lysate; (2) MCF-7 cell lysate.
Specific Publications For Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody (P00006)
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4 Customer Q&As for Anti-Phospho-PTEN (S380) Rabbit Monoclonal Antibody
Question
My colleagues were happy with the WB result of your anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody. However we have seen positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is PTEN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
From literature, liver does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-07
Question
We ordered your anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody for WB on lung a few years ago. I am using human, and We want to use the antibody for IF next. We are interested in examining lung as well as spleen in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
I looked at the website and datasheets of our anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody and it seems that P00006 has been tested on human in both WB and IF. Thus P00006 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody P00006 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody (P00006) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-01
Question
We have seen staining in mouse liver. What should we do? Is anti-Phospho-PTEN (S380) Rabbit Monoclonal antibody supposed to stain liver positively?
C. Mitchell
Verified customer
Asked: 2013-04-19
Answer
According to literature liver does express PTEN. According to Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-04-19
