Click image to see more details
Product Info Summary
| SKU: | M00006-3 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265
SKU/Catalog Number
M00006-3
Size
100uL
Form
Liquid
Description
Boster Bio Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 (Catalog # M00006-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM265
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human PTEN
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and has dual-specificity to protein phosphatase PTEN. This antibody may also react to mouse PTEN, as predicted by immunogen homology.
Reactive Species
M00006-3 is reactive to PTEN in Human
Applications
M00006-3 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47.166kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa and 293 cells lysates using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-PTEN Rabbit Monoclonal Antibody (Clone RM265) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For PTEN (Source: Uniprot.org, NCBI)
Gene Name
PTEN
Full Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Weight
47.166kDa
Superfamily
PTEN phosphatase protein family
Alternative Names
BZS; EC 3.1.3.16,10q23del; EC 3.1.3.48; EC 3.1.3.67; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; MMAC1; MMAC1MGC11227; Mutated in multiple advanced cancers 1; phosphatase and tensin homologDEC; phosphatidylinositol-34,5-trisphosphate 3-phosphatase and dual-specificityprotein phosphatase PTEN; PTEN; PTEN1; TEP1MHAM PTEN 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC11, PTENbeta, TEP1, PTEN phosphatase and tensin homolog phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|MMAC1 phosphatase and tensin homolog deleted on chromosome 10|PTENepsilon|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|protein tyrosine phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTEN, check out the PTEN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTEN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265 (M00006-3)
Hello CJ!
M00006-3 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Aberrant PTEN, PIK3CA, pMAPK, and TP53 expression in human scalp and face angiosarcoma
Identification and profiling of microRNAs expressed in oral buccal mucosa squamous cell carcinoma of Chinese hamster
Sun M,Hu L, S,Huang T,Zhang M,Yang M,Zhen W,Yang D,Lu W,Guan M,Peng S. Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation. J Bone Miner Res.2020 Feb;35(2):306-316. doi:10.1002/jbmr.3892.
Species: Human,Mouse
M00006-3 usage in article: APP:WB, SAMPLE:HMSCS CELL AND MC3T3-E1 CELL, DILUTION:1:500
Long-term effects of repeated superovulation on the uterus and mammary gland in rhesus monkeys
miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit
miR-21 increases c-kit cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling
Expression and significance of PTEN, hypoxia-inducible factor-1 alpha in colorectal adenoma and adenocarcinoma
Prognostic role of PPAR-%u03B3 and PTEN in the renal cell carcinoma
708: Combined Analysis of EGFR and PTEN Status in Patients With KRAS Wild-Type Metastatic Colorectal Cancer
Overexpression of LRIG1 regulates PTEN via MAPK/MEK signaling pathway in esophageal squamous cell carcinoma
Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PTEN Rabbit Monoclonal Antibody, Clone#RM265
Question
I was wanting to use your anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 for IHC for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
As indicated on the product datasheet, M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-27
Question
My question regarding product M00006-3, anti-PTEN Rabbit Monoclonal antibody, Clone#RM265. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3. Let me know if you need anything else.
K. Dhar
Verified customer
Asked: 2020-02-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
We are currently using anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 (M00006-3) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-06
Question
Does M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
It shows on the product datasheet, M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-31
Question
Would anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 work for IHC with lung?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
According to the expression profile of lung, PTEN is highly expressed in lung. So, it is likely that anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 will work for IHC with lung.
Boster Scientific Support
Answered: 2020-01-14
Question
Is a blocking peptide available for product anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 (M00006-3)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We do provide the blocking peptide for product anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 (M00006-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-23
Question
We have been able to see staining in mouse spleen. Do you have any suggestions? Is anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 supposed to stain spleen positively?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
From literature spleen does express PTEN. From Uniprot.org, PTEN is expressed in intestine, epithelium, spleen, lung, liver, among other tissues. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-11-12
Question
I was wanting to use to test anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 on mouse lung for research purposes, then I may be interested in using anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The products we sell, including anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-24
Question
We were content with the WB result of your anti-PTEN Rabbit Monoclonal antibody, Clone
D. Jones
Verified customer
Asked: 2019-07-10
Answer
From literature, intestine does express PTEN. Generally PTEN expresses in cytoplasm. Regarding which tissues have PTEN expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 9187108, 9090379
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-10
Question
Is there a BSA free version of anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 available?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-21
Question
I see that the anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-13
Question
I have attached the WB image, lot number and protocol we used for lung using anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 M00006-3. Please let me know if you require anything else.
R. Walker
Verified customer
Asked: 2017-07-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-13
Question
Is this M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 reactive to the isotypes of PTEN?
C. Baker
Verified customer
Asked: 2017-05-05
Answer
The immunogen of M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 is A peptide corresponding to the C-terminus of human PTEN. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-05
Question
I am interested in using your anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 for long-term synaptic depression studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.
T. Miller
Verified customer
Asked: 2013-11-22
Answer
I appreciate your inquiry. This M00006-3 anti-PTEN Rabbit Monoclonal antibody, Clone#RM265 is validated on 293 cells, human brain tissue. It is guaranteed to work for IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-11-22
Question
We purchased anti-PTEN Rabbit Monoclonal antibody, Clone
N. Williams
Verified customer
Asked: 2013-07-01
Answer
I looked at the website and datasheets of our anti-PTEN Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2013-07-01
