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Product Info Summary
| SKU: | P00018-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00018-1
BM4507 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody catalog # P00018-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00018-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FDA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-SIRT1 (S47)
Reactive Species
P00018-1 is reactive to SIRT1 in Human, Mouse, Rat
Observed Molecular Weight
110 kDa
Calculated molecular weight
81.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00018-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HEK293 cell, HEK293 cell
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Phospho-SIRT1 (S47) expression in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.
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Proteins expressions of SIRT1-AMPK-FOXO3 pathway and autophagy markers in C28/I2 chondrocytes after 4-hour T-2 toxin treatment. T5: 5 ng/mL T-2 toxin; T20: 20 ng/mL T-2 toxin. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40763514
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Proteins expressions of SIRT1-AMPK-FOXO3 pathway and autophagy markers of C28/I2 chondrocytes treated with 20 ng/mL T-2 toxin and 100 ng/mL CSA-SeNP for 12 h. ST: Treatment separately with T-2 toxin; SC: Treatment separately with CSA-SeNP; TC: Treatment with T-2 toxin and CSA-SeNP. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40763514
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IHC analysis of P-SIRT1 using anti-P-SIRT1 antibody (P00018-1).
P-SIRT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-SIRT1 Antibody (P00018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of P-SIRT1 using anti-P-SIRT1 antibody (P00018-1).
P-SIRT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-SIRT1 Antibody (P00018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of P-SIRT1 using anti-P-SIRT1 antibody (P00018-1).
P-SIRT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-SIRT1 Antibody (P00018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of P-SIRT1 using anti-P-SIRT1 antibody (P00018-1).
P-SIRT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-SIRT1 Antibody (P00018-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody (P00018-1)
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4 Customer Q&As for Anti-Phospho-SIRT1 (S47) Rabbit Monoclonal Antibody
Question
We bought anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody for WB on prostate in a previous project. I am using human, and We are going to use the antibody for IF next. I would like examining prostate as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I have checked the website and datasheets of our anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody and it seems that P00018-1 has been validated on human in both WB and IF. Thus P00018-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-26
Question
We are currently using anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody P00018-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody (P00018-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
My colleagues were well pleased with the WB result of your anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody. However we have seen positive staining in liver nucleus using this antibody. Is that expected? Could you tell me where is SIRT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Based on literature, liver does express SIRT1. Generally SIRT1 expresses in nucleus, pml body, sirtt1 75 kda fragment: cytoplasm. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2019-06-07
Question
We have seen staining in human right adrenal gland. Do you have any suggestions? Is anti-Phospho-SIRT1 (S47) Rabbit Monoclonal antibody supposed to stain right adrenal gland positively?
R. Mangal
Verified customer
Asked: 2014-07-17
Answer
Based on literature right adrenal gland does express SIRT1. Based on Uniprot.org, SIRT1 is expressed in right adrenal gland, testis, prostate, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Prostate, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2014-07-17
