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Product Info Summary
| SKU: | M05079-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Aldehyde dehydrogenase 10 (ALDH3A2) Mouse Monoclonal Antibody [Clone ID: OTI2A7]
SKU/Catalog Number
M05079-2
Size
100 µl
Description
Boster Bio ALDH3A2 mouse monoclonal antibody, clone OTI2A7 (formerly 2A7). Catalog# M05079-2. Tested in FC, IHC, WB. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Aldehyde dehydrogenase 10 (ALDH3A2) Mouse Monoclonal Antibody [Clone ID: OTI2A7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05079-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A7
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human ALDH3A2 (NP_001026976) produced in HEK293T cell.
Reactive Species
M05079-2 is reactive to ALDH3A2 in Human, Rat
Calculated molecular weight
54.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05079-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IHC 1:150
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH3A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2.
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ALDH3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ALDH3A2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH3A2 antibody (M05079-2)
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ALDH3A2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ALDH3A2 monoclonal antibody M05079-2 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-Aldehyde dehydrogenase 10 (ALDH3A2) Mouse Monoclonal Antibody [Clone ID: OTI2A7] (M05079-2)
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