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- Table of Contents
PIK3R1 antibody (M00318-2) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
PIK3R1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
PIK3R1 antibody was positively detected by WB in:
human Jurkat whole cell, human K562 whole cell, human 293T whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ICC
IF
Human
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Product Information
Product Name
Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
View all PIK3R1 Antibodies
View all PIK3R1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17P38
Isotype
IgG
Immunogen
A synthesized peptide derived from human PI3 Kinase p85 alpha
Observed Molecular Weight
84 kDa
Calculated Molecular Weight
83.6 kDa
Cite This Product
Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00318-2)
Protocols
Western Blot protocol for PIK3R1 antibody
Publications
Application:
Species:
AB4-Loaded Nanomicelle Hydrogel Promotes Targeting of the Dysregulated Diabetic Wound Microenvironment via Coordinated Multistage Repair
Published: 2026-08-14
Journal: Gels
Application: WB
Impact Factor: 6.40
Species: Mouse
PMID: 42644966
Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation
Published: 2026-02-21
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 8.30
Species: Mouse
PMID: 41759424
Safflower washing medicine alleviating acute soft tissue injury via PI3K/Akt pathway
Published: 2026-01-16
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 41548617
The Combination of Phytic Acid and Inositol Alleviates Metastasis of Colorectal Cancer in Mice by Inhibiting PI3K/AKT Pathway and M2 Macrophage Polarization
Published: 2025-12-02
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.70
Species: Mouse
PMID: 41339008
Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells
Published: 2025-11-29
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 41325790
Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment
Published: 2025-07-14
Journal: Cancer Nanotechnology
Application: WB
Impact Factor: 4.80
Species: Human
Transcriptome analysis reveals that the injection of mesenchymal stem cells remodels extracellular matrix and complement components of the brain through PI3K/AKT/FOXO1 signaling pathway in a neuroinflammation mouse model
Published: 2025-03-21
Journal: GENOMICS
Application: WB
Impact Factor: 3.00
Species: Mouse
PMID: 40122474
Preconditioning with acteoside ameliorates myocardial ischemia‑reperfusion injury by targeting HSP90AA1 and the PI3K/Akt signaling pathway
Published: 2025-01-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 3.50
Species: Rat
PMID: 39886969
Curcumin-enhanced antitumor effects of sorafenib via regulating the metabolism and tumor microenvironment
Published: 2020-06-20
Journal: Food & Function
Application: WB
Impact Factor: 4.17
Species: Mouse
PMID: 32613952
Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway
Published: 2019-12-05
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 3.85
Species: Mouse
PMID: 31920652
Mulberry Bark Alleviates Effect of STZ Inducing Diabetic Mice through Negatively Regulating FoxO1
Published: 2019-01-21
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.98
Species: Mouse
PMID: 30800168
Review
Have you used Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody?
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3 Reviews For Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody
Anti-PI3K p85α Antibody (M00318-2) produced sharp target bands at the predicted molecular weight with negligible background.
| SKU | M00318-2 |
|---|---|
| Application | Western Blot |
| Sample | PC-12 (rat adrenal pheochromocytoma cells, highly differentiated) |
| Sample Processing Description | ① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | PI3 Kinase p85 alpha Rabbit Monoclonal Antibody |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed that the target band was clear, the total PI3K protein expression was downregulated after modeling, and the expression level rebounded after drug treatment. |
Yue Zhang, Chengdu University of Traditional Chinese Medicine
Verified customer
Submitted 2026-08-26
Anti-PI3K p85α Antibody (M00318-2) produced sharp target bands at the predicted molecular weight with negligible background. WB data revealed reduced total PI3K p85α expression in Aβ-induced PC-12 AD model cells, and drug treatment partially restored its
| SKU | M00318-2 |
|---|---|
| Application | Western Blot |
| Sample | PC-12 (rat adrenal pheochromocytoma cells, highly differentiated) |
| Sample Processing Description | ① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | PI3 Kinase p85 alpha Rabbit Monoclonal Antibody |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | ① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed that the target band was clear, the total PI3K protein expression was downregulated after modeling, and the expression level rebounded after drug treatment. |
Yue Zhang, Chengdu University of Traditional Chinese Medicine
Verified customer
Submitted 2026-08-26
The PI3K p85 alpha Antibody (M00318-2) showed clear and specific bands in WB of rat skeletal muscle, with PI3K p85 expression increased in the model group and decreased in a dose-dependent manner in the treatment groups, consistent with expectations.
| SKU | M00318-2 |
|---|---|
| Application | Western Blot |
| Sample | Rat skeletal muscle tissue |
| Sample Processing Description | Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer,Blocking buffer |
| Primary Antibody | PI3 Kinase p85 alpha Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system: ChemiDoc MP |
| Results Summary | Based on the experimental results, the band of PI3K p85 is clear. The PI3K p85 level in the model group is significantly higher than in the normal group, whereas in the treatment group, the PI3K p85 level decreases with increasing drug dose, which is consistent with expectations. |
Guangtian Yu, Ningxia Medical University
Verified customer
Submitted 2026-03-26
Product Promise
We promise Anti-PI3 Kinase p85 alpha Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00318-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026