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Product Info Summary
| SKU: | A00748-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
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Product info
Product Name
Anti-MIP-3 Alpha/CCL20 Antibody Picoband®
SKU/Catalog Number
A00748-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIP-3 Alpha/CCL20 Antibody Picoband® catalog # A00748-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIP-3 Alpha/CCL20 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00748-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse MIP-3 Alpha/CCL20 recombinant protein (Position: A28-M97).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00748-2 is reactive to Ccl20 in Mouse, Rat
Observed Molecular Weight
14 kDa
Calculated molecular weight
10.8 kDa
Background of Ccl20
Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00748-2 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.
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Sandwich ELISA - Recombinant mouse MIP-3 Alpha/CCL20 protein standard curve.
Use in combination with reagents from Mouse MIP-3 Alpha/CCL20 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0454).
Specific Publications For Anti-MIP-3 Alpha/CCL20 Antibody Picoband® (A00748-2)
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4 Customer Q&As for Anti-MIP-3 Alpha/CCL20 Antibody Picoband®
Question
Can you help my question with product A00748-2, anti-MIP-3 Alpha/CCL20 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00748-2 anti-MIP-3 Alpha/CCL20 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
We are currently using anti-MIP-3 Alpha/CCL20 antibody A00748-2 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The anti-MIP-3 Alpha/CCL20 antibody (A00748-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-10
Question
Would anti-MIP-3 Alpha/CCL20 antibody A00748-2 work for ELISA with pancreas?
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
According to the expression profile of pancreas, CCL20 is highly expressed in pancreas. So, it is likely that anti-MIP-3 Alpha/CCL20 antibody A00748-2 will work for ELISA with pancreas.
Boster Scientific Support
Answered: 2018-09-19
Question
Is a blocking peptide available for product anti-MIP-3 Alpha/CCL20 antibody (A00748-2)?
J. Patel
Verified customer
Asked: 2013-06-13
Answer
We do provide the blocking peptide for product anti-MIP-3 Alpha/CCL20 antibody (A00748-2). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-06-13
