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Product Info Summary
| SKU: | PA1410 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
SKU/Catalog Number
PA1410
BA1352-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody catalog # PA1410. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1410)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PI 3 Kinase p85 alpha, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1410 is reactive to PIK3R1 in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
83.6 kDa
Background of PIK3R1
Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1410 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: MCF-7 Cell, HELA Cell, COLO-320 whole cell, SW620 Cell,, rat brain tissue, rat lung tissue, rat PC-12 whole cell, mouse brain tissue, mouse HEPA1-6 whole cell, mouse RAW2467 whole cell
Validation Images & Assay Conditions
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Anti-PI 3 Kinase p85 alpha antibody, PA1410, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: COLO-320 whole cell lysate
Lane 4: SW620 Cell Lysate
Click image to see more details
Western blot analysis of PI 3 Kinase p85 alpha using anti-PI 3 Kinase p85 alpha antibody (PA1410).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates
Lane 2: rat lung tissue lysates
Lane 3: rat PC-12 whole cell lysates
Lane 4: mouse brain tissue lysates
Lane 5: mouse HEPA1-6 whole cell lysates
Lane 6: mouse RAW246.7 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha antigen affinity purified polyclonal antibody (Catalog # PA1410) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha at approximately 85KD. The expected band size for PI 3 Kinase p85 alpha is at 85KD.
Specific Publications For Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® (PA1410)
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5 Customer Q&As for Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Question
We are currently using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (PA1410) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
Does PA1410 anti-PI 3 Kinase p85 alpha/PIK3R1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-08
Answer
It shows on the product datasheet, PA1410 anti-PI 3 Kinase p85 alpha/PIK3R1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-08
Question
What COLO cell lysate was used for WB testing of PA1410?
Verified customer
Asked: 2019-03-30
Answer
COLO-320 whole cell lysate was used in WB for the Anti-PI 3 Kinase P85 Alpha/PIK3R1 Antibody (PA1410).
Boster Scientific Support
Answered: 2019-04-02
Question
I see that the anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 works with WB, what is the protocol used to produce the result images on the product page?
D. Brown
Verified customer
Asked: 2019-01-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-01-08
Question
Will anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 work on primate WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
Our lab technicians have not tested anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 on primate. You can run a BLAST between primate and the immunogen sequence of anti-PI 3 Kinase p85 alpha/PIK3R1 antibody PA1410 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate skeletal muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-14
