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PIK3CA antibody (PB9314) performs well in Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Flow Cytometry analysis of Raji cells using anti-PIK3CA antibody (PB9314).
Overlay histogram showing Raji cells stained with PB9314 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3CA Antibody (PB9314, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Raji cells stained with PB9314 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3CA Antibody (PB9314, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband®
PIK3CA antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, WB |
Tested Applications
PIK3CA antibody was positively detected by WB in:
human Jurkat whole cell, human MCF-7 whole cell, human K562 whole cell, human Raji whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PIK3CA antibody was positively detected by FC in:
Raji cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Mouse
Predicted
Rat
Predicted
Zebrafish
Predicted
Product Information
Product Name
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband®
View all PIK3CA Antibodies
View all PIK3CA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Clone Number
WH-3
Immunogen
E.coli-derived human PIK3CA recombinant protein (Position: H936-N1068). Human PIK3CA shares 98% amino acid (aa) sequence identity with mouse PIK3CA.
Cross-Reactivity
No cross reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
124 kDa
Calculated Molecular Weight
124.3 kDa
Background of PIK3CA
Phosphatidylinositol-4,5-bisphosphate 3-kinase, also called PIK3CA, is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. PIK3CA gene is mapped to 3q26.32. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate phosphatidylinositols (PtdIns), PtdIns4P andPtdIns (4,5)P2. Recent evidence has shown that the PIK3CA gene is mutated in a range of human cancers. It has been found to be oncogenic and has been implicated in cervical cancers. PIK3CA mutations in breast cancer may be a predictive marker to guide the selection of patients who would benefit from mTOR inhibitor therapy. In addition to that, the presence of PIK3CA mutation may predict response to aspirin therapy for colorectal cancer, indicating power and promise of "Molecular Pathological Epidemiology (MPE)" approach as well as a complex interaction within the tumor microenvironment in this phenomenon.
Cite This Product
Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9314)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
98%
High Homology
Rat
98%
High Homology
Zebrafish
96%
High Homology
Drosophila
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PIK3CA antibody
Flow Cytometry (FC) protocol for PIK3CA antibody
Publications
Application:
Species:
Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling
Published: 2024-08-10
Journal: Cancers
Application: WB
Impact Factor: 4.50
Species: Human
PMID: 39199589
The mechanism study of inhibition effect of prepared Radix Rehmanniainon combined with Radix Astragali osteoporosis through PI3K-AKT signaling pathway
Published: 2023-01-06
Journal: Acta Cirurgica Brasileira
Application: IHC
Impact Factor: 1.10
Species: Rat
PMID: 36629528
Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation
Published: 2022-07-26
Journal: Journal of Integrative Medicine-JIM
Application: WB,IF
Impact Factor: 3.95
Species: Human
PMID: 35965234
miR‑320a in serum exosomes promotes myocardial fibroblast proliferation via regulating the PIK3CA/Akt/mTOR signaling pathway in HEH2 cells
Published: 2021-06-14
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 2.45
Species: Human
PMID: 34194551
Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology
Published: 2021-05-06
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.81
Species: Rat
PMID: 34025396
Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway
Published: 2020-12-31
Journal: MEDICAL SCIENCE MONITOR
Application: WB
Impact Factor: 1.92
Species: Human
PMID: 33382670
Circular RNA hsa_circ_0010882 promotes the progression of gastric cancer via regulation of the PI3K/Akt/mTOR signaling pathway.
Published: 2020-02-01
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 3.02
Species: Human
PMID: 32096170
Insulin enhancement of the antitumor activity of chemotherapeutic agents in colorectal cancer is linked with downregulating PIK3CA and GRB2
Published: 2019-11-12
Journal: Scientific Reports
Application: WB
Impact Factor: 4.01
Species: Human
PMID: 31719636
Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway
Published: 2019-10-16
Journal: OncoTargets and Therapy
Application: WB
Impact Factor: 3.05
Species: Human
PMID: 31802893
Up-regulated circular RNA hsa_circ_0067934 contributes to glioblastoma progression through activating PI3K-AKT pathway
Published: 2019-04-30
Journal: European Review for Medical and Pharmacological Sciences
Application: WB
Impact Factor: 2.72
Species: Human
PMID: 31081099
ZWINT is the next potential target for lung cancer therapy
Published: 2019-01-14
Journal: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 30643969
The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats
Published: 2016-08-31
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 4.65
Species: Rat
PMID: 27576607
Abnormal expression of PTEN and PIK3CA in pemetrexed-resistant human pancreatic cancer cell line Patu8988.
Published: 2016-07-29
Journal: GENETICS AND MOLECULAR RESEARCH
Application: WB
Impact Factor: 0.76
Species: Human
PMID: 27525871
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Product Promise
We promise Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9314
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026